DNA Electrophoresis

Methods and Protocols

Buch | Softcover

228 Seiten

2021 | 1st ed. 2020
Springer-Verlag New York Inc.
978-1-0716-0325-3 (ISBN)

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This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field.

Introduction and Perspectives of DNA Electrophoresis.- Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers.- Using Two-Dimensional Intact Mitochondrial DNA (mtDNA) Agarose Gel Electrophoresis (2D-IMAGE) to Detect Changes in Topology Associated with Mitochondrial Replication, Transcription, and Damage.- 2D Gel Electrophoresis to Detect DNA Replication and Recombination Intermediates in Budding Yeast.- Neutral-Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures.- Alkali Comet Assay in Genotoxicity Tests.- The Analysis of DNA Interstrand Crosslinks and their Repair by the Modified Comet Assay.- Analysis of Chromosomal DNA Fragmentation in Apoptosis by Pulsed-Field Gel Electrophoresis.- Detection of DNA Damage-Induced DSBs by the Contour-Clamped Homogeneous Electric Field (CHEF) System in Mammalian Cells.- Investigation of DNA Double-Strand Breaks Induced in Host Cells following Infection with Genotoxic Bacteria.- Monitoring of DNA Replication and DNA Double-Strand Breaks in Saccharomyces cerevisiae by Pulsed-Field Gel Electrophoresis (PFGE).- Pulsed-Field Gel Electrophoresis for Detecting Chromosomal DNA Breakage in Fission Yeast.- Detection of DNA Double-Strand Breaks by Pulsed-Field Gel Electrophoresis of Circular Bacterial Chromosomes.- Detection of Bleomycin-Induced DNA Double-Strand Breaks in Escherichia coli by Pulsed-Field Gel Electrophoresis using a Rotating Gel Electrophoresis System.- Circle-Seq: Isolation and Sequencing of Chromosome-Derived Circular DNA Elements in Cells.- Chromatin Pull-Down Methodology Based on DNA Triple Helix Formation.- DNA Fragments Agarose Gel Electrophoresis for Chromatin Immunoprecipitation (ChIP).- Post-Labeling/PAGE Method for Detection of DNA Adducts.

Erscheinungsdatum	05.02.2021
Reihe/Serie	Methods in Molecular Biology ; 2119
Zusatzinfo	40 Illustrations, color; 30 Illustrations, black and white; X, 228 p. 70 illus., 40 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Medizin / Pharmazie ► Physiotherapie / Ergotherapie ► Orthopädie
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Technik ► Medizintechnik
Schlagworte	DNA replication forks • Genotoxicology • mitochondrial DNA replication • pulsed-field gel electrophoresis • Thermogradient gel electrophoresis
ISBN-10	1-0716-0325-6 / 1071603256
ISBN-13	978-1-0716-0325-3 / 9781071603253
Zustand	Neuware