Bioluminescent Imaging

Methods and Protocols

Buch | Softcover

246 Seiten

2020 | 1st ed. 2020
Humana Press Inc. (Verlag)
978-1-4939-9942-2 (ISBN)

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This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols to ensure successful results in the further study of this vital field.

High-throughput Bioluminescence Imaging and Reporter Gene Assay with 3D Spheroids from Human Cell Lines.- Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cells.- High-throughput Analysis of Endocrine Disrupting Compounds using BLYES and BLYAS Bioluminescent Yeast Bioassays.- Bioluminescent Imaging of Single Bacterial Cells Using an Enhanced ilux Operon.- Bioluminescent Imaging and Tracking of Bacterial Transport in Soils.- In vivo Bioluminescent Imaging of Yersinia ruckeri Pathogenesis in Fish.- In Vivo Bioluminescent Monitoring of Parasites in BALB/c Mouse Models of Cutaneous Leishmaniasis Drug Discovery.- Multiplex Imaging of Polymicrobial Communities – Murine Models to Study Oral Microbiome Interactions.- Bioluminescence Imaging to Study Mature Biofilm Formation by Candida spp. and Antifungal Activity in vitro and in vivo.- Bioluminescence Imaging of Neuroinflammation in a Mouse Model of Parkinson’s Disease.- Continual Conscious Bioluminescent Imaging in Freely Moving Mice.- In Vivo Bioluminescent Imaging of Marburg Virus in a Rodent Model.- Continuous and Real-time In Vivo Autobioluminescent Imaging in a Mouse Model.- In vivo Tracking of Tumor-derived Bioluminescent Extracellular Vesicles in Mice.- Bioluminescence Imaging in the Chick Chorioallantoic Membrane Assay.- Application of a cybLuc Aminoluciferin for Deep Tissue Bioluminescence Imaging in Rodent Models.- The Application of Single Cell Bioluminescent Imaging to Monitor Circadian Rhythms of Individual Plant Cells.

Erscheinungsdatum	20.11.2020
Reihe/Serie	Methods in Molecular Biology ; 2081
Zusatzinfo	63 Illustrations, color; 21 Illustrations, black and white; XI, 246 p. 84 illus., 63 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizinische Fachgebiete ► Radiologie / Bildgebende Verfahren ► Radiologie
Themenwelt	Technik ► Umwelttechnik / Biotechnologie
Schlagworte	Alligator • bacterial trafficking • Caenorhabditis elegans • fuel • luciferases
ISBN-10	1-4939-9942-7 / 1493999427
ISBN-13	978-1-4939-9942-2 / 9781493999422
Zustand	Neuware