Vaccine Delivery Technology
Springer-Verlag New York Inc.
978-1-0716-0794-7 (ISBN)
Cutting-edge and practical, Vaccine Delivery Technology: Methods and Protocols is a valuable resource for both novice and expert researchers, in and outside the field, who would like to gain insight into the impactful fieldof vaccines.
Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Vaccine Delivery and Immune Response Basics.- Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) using Bacterial Whole Cells as Molecular Probes.- Use of Chlamydial Elementary Bodies as probes to Isolate Pathogen-Specific Human Monoclonal Antibodies.- Computational Antigen Discovery for Eukaryotic Pathogens using Vacceed.- Antigen Discovery in Bacterial Pan-Proteomes.- Alphavirus-Based Antigen Preparation.- Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells.- Bioreactor-Based Antigen Production Process using the Baculovirus Expression Vector System.- High-Throughput Process Development for the Chromatographic Purification of Viral Antigens.- Production of Zika Virus Virus-Like Particles.- Biosynthesis of Glycoconjugate Virus-Like Particles (VLPs).- Up- and Downstream Processes for Viral Nanoplaxes as Vaccines.- Whole-Cell Vaccine Preparation: Options and Perspectives.- O-AntigenExtraction, Purification, and Chemical Conjugation to a Carrier Protein.- Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines.- Exploitation of Capsule Polymerases for the Enzymatic Synthesis of the Polysaccharide Antigens used in Glycoconjugate Vaccines.- Attenuation Methods for Live Vaccines.- Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism.- Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells.- Lipofection-Based Delivery of DNA Vaccines.- Flavivirus DNA Vaccine Design and Adjuvant Selection.- Vaccine Delivery with a Detoxified Bacterial Toxin.- Needle-Less or Non-Invasive Delivery Technology.- Lactic Acid Bacteria as Delivery Vehicle for Therapeutics Applications.- A Hybrid Biological-Biomaterial Vector for Antigen Delivery.- Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens.- Thin-Film Freeze-Drying is a Viable Method to Convert Vaccines Containing Aluminum Salts from Liquid to Dry Powder.- The Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers.- Methods to Evaluate Immune Cell Recruitment and the Cellular Uptake and Distribution of Antigen following Intramuscular Administration of Vaccine to Mice.- The Quantification of Antigen-Specific T Cells by IFN-γ ELISpot.- The Measurement of Antigen-Specific IgG Titers by Direct ELISA.- Method to Evaluate In Vivo CD8+ T Cell Cytotoxicity in a Murine Model.- Testing Anti-Pneumococcal Antibody Function using Bacteria and Primary Neutrophils.- Viral Infectivity Qunatification and Neutralization Assays using Laser Force Cytology.
| Erscheinungsdatum | 25.09.2020 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 2183 |
| Zusatzinfo | 127 Illustrations, color; 22 Illustrations, black and white; XV, 590 p. 149 illus., 127 illus. in color. |
| Verlagsort | New York, NY |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Chemie |
| Technik ► Maschinenbau | |
| Technik ► Umwelttechnik / Biotechnologie | |
| ISBN-10 | 1-0716-0794-4 / 1071607944 |
| ISBN-13 | 978-1-0716-0794-7 / 9781071607947 |
| Zustand | Neuware |
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