Multi-Parametric Live Cell Microscopy of 3D Tissue Models

Buch | Softcover

XII, 172 Seiten

2018 | 1. Softcover reprint of the original 1st ed. 2017
Springer International Publishing (Verlag)
978-3-319-88418-9 (ISBN)

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This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.

Ruslan I. Dmitriev graduated from Lomonosov Moscow State Academy of Fine Chemical Technology (MSc) and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (PhD, 2008), where he studied membrane ion-transporting proteins and protein interactions. He trained as postdoc at University College Cork, focusing on cell metabolism, hypoxia research and development and biological applications of cell-penetrating phosphorescent probes for molecular oxygen. Since 2014, he leads Metabolic Imaging group at University College Cork, where he designs novel biocompatible FLIM and PLIM biosensors for 3D tissue models for regenerative medicine and cancer biology.

Current state-of-the-art 3D tissue models and their compatibility with live cell imaging.- Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation.- Quantitative live cell FLIM imaging in three dimensions.- Three-dimensional tissue models and available probes for multi-parametric live cell microscopy: a brief overview.- Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues.- Multi-parametric imaging of hypoxia and cell cycle in intestinal organoid culture.- Imaging of intracellular pH in tumor spheroids using genetically encoded sensor SypHer2.- Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell.- Quantitative imaging of Ca2+ by 3D-FLIM in live tissues.- Live cell imaging of viscosity in 3D tumour cell models.- Live imaging of cell invasion using a multicellular spheroid model and lightsheet microscopy.- Raman imaging microscopy for quantitative analysis of biological samples.

Erscheint lt. Verlag	23.6.2018
Reihe/Serie	Advances in Experimental Medicine and Biology
Zusatzinfo	XII, 172 p. 53 illus., 39 illus. in color.
Verlagsort	Cham
Sprache	englisch
Maße	178 x 254 mm
Gewicht	361 g
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Biomedizin
	Medizin / Pharmazie ► Physiotherapie / Ergotherapie ► Orthopädie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
	Technik ► Medizintechnik
Schlagworte	3D live cell imaging • 3D tissue models • Applications Multi-parametric imaging • Autofluorescence imaging in cancer • Biological Microscopy • Ca2+ imaging neural • Multi-parametric imaging cancer • Multi-parametric imaging of pH • Multi-parametric imaging viscosity cancer • Multi-parametric quantitative imaging • Quantitative imaging intestinal organoid
ISBN-10	3-319-88418-2 / 3319884182
ISBN-13	978-3-319-88418-9 / 9783319884189
Zustand	Neuware