Study of the Calcium Regulation Mechanism of TCR Activation Using Nanodisc and NMR Technologies

Yunchen Bi (Autor)

Buch | Softcover

XII, 79 Seiten

2018 | 1. Softcover reprint of the original 1st ed. 2018
Springer Berlin (Verlag)
978-3-662-57206-1 (ISBN)

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This thesis describes the use of biophysical and biochemical methods to prove that calcium has a positive feedback effect on amplifying and sustaining CD3 phosphorylation and should enhance T-cell sensitivity to foreign antigens. The study presented shows that calcium can regulate the signal pathway in cells not only as a secondary messenger but also through direct interactions with the phospholipid bilayer. The approach used in the thesis also represents an important advance, as it couples the use of nuclear magnetic resonance (NMR) to the analysis of signaling phenomena in living cells. Moreover, the thesis optimizes the Nanodisc assembly protocol, which can broaden its range of applications in membrane protein studies. A preliminary study on the structure of dengue virus NS2B-NS3p in complex with aprotinin, which may help to develop new drugs against the dengue virus, is also included.

Introduction.- Review.- Nanodisc assembly and the study on the interaction between the lipid and ion.- Calcium regulates T cell receptor activation through phospholipid electrostatic manipulation.- The preliminary study on the structure of Dengue Virus NS2B-NS3p in complex with aprotinin.- Summary and prospect of this dissertation.

Erscheinungsdatum	05.03.2022
Reihe/Serie	Springer Theses
Zusatzinfo	XII, 79 p. 41 illus.
Verlagsort	Berlin
Sprache	englisch
Maße	155 x 235 mm
Gewicht	1533 g
Themenwelt	Medizin / Pharmazie ► Physiotherapie / Ergotherapie ► Orthopädie
Themenwelt	Technik
Schlagworte	Calcium regulation • Dengue virus • Nanodisc technology • NMR • Nuclear Magnetic Resonance • Protein-Lipid interaction • T cell receptor • TCR
ISBN-10	3-662-57206-0 / 3662572060
ISBN-13	978-3-662-57206-1 / 9783662572061
Zustand	Neuware