Hydrocarbon and Lipid Microbiology Protocols

Isolation and Cultivation

Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales (Herausgeber)

Buch | Softcover

X, 319 Seiten

2018 | 1. Softcover reprint of the original 1st ed. 2017
Springer Berlin (Verlag)
978-3-662-56878-1 (ISBN)

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This Volume describes methods for cultivating hydrocarbon-producing and -consuming microbes, covering compounds in a range of states - gaseous (e.g. methane), liquid (e.g. alkanes of intermediate molecular weight) and solid (e.g. many PAHs and asphaltene). It also examines the cultivation of aerobic and anaerobic hydrocarbon degraders using a range of electron acceptors (e.g. oxygen, nitrate, sulphate, metals, (per)chlorate), and a separate chapter is devoted to explaining the cultivation of methanogens. Special attention is given to: high-throughput cultivation, growing microbes as biofilms, and cultivating fastidious microbes, as well as the preservation of microbial pure cultures and consortia. Accordingly, this Volume will be of value to anyone embarking on the selective enrichment and cultivation of novel microorganisms.
Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Introduction to the cultivation of microbes involved in the cycling of hydrocarbons.- Cultivating fastidious microbes.- The essentials of cultivating aerobic hydrocarbon-degrading microbes.- Cultivating aerobic hydrocarbon-degrading microbes from microalgae.- Growing and maintaining hydrocarbon-degrading consortia.- Two-phase cultivation techniques for hydrocarbon-degrading organisms.- Cultivation of hydrocarbon-degrading fungi.- Growth of hydrocarbon-degrading bacteria in continuous culture.- Growth procedures at low substrate concentrations.- Cultivation of biofilm-forming hydrocarbon degraders.- Cultivation of asphaltene-degrading microbes.- Cultivation of microorganisms for fuel quality improvement.- Simulation of oxygen and redox oscillations in crude oil- degrading bioreactors.- Cultivation of anaerobic microorganisms with hydrocarbons as growth substrates.- Enrichment and isolation of metal respirers and hydrocarbon oxidizers.- Enrichment and isolation of oxyion-respiring hydrocarbon oxidizers.- Cultivation of methanogens.- Cultivation of methylotrophs.- Cultivation of methanotrophs.- Cultivation of extremophilic hydrocarbon degraders.- Cultivation of piezophilic hydrocarbon degraders.- Cultivation of oil-producing microalgae.- Cultivation of oleaginous yeasts.- Cultivation of oil-tolerant protists.

Erscheinungsdatum	05.03.2022
Reihe/Serie	Springer Protocols Handbooks
Zusatzinfo	X, 319 p. 50 illus., 20 illus. in color.
Verlagsort	Berlin
Sprache	englisch
Maße	178 x 254 mm
Gewicht	633 g
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Themenwelt	Technik ► Umwelttechnik / Biotechnologie
Schlagworte	Asphaltene-degrading microbes • biochemical engineering • Biofilm-forming microbes • Crude oil-degradation • Fuel quality improvement • Hydrocarbon-degrading microbes • Hydrocarbons as growth substrates • Methylotrophs • Oil-degrading bioreactors • Oil-producing microalgae
ISBN-10	3-662-56878-0 / 3662568780
ISBN-13	978-3-662-56878-1 / 9783662568781
Zustand	Neuware