Understanding Light Microscopy

J Sanderson (Autor)

Software / Digital Media

848 Seiten

2019
John Wiley & Sons Inc (Hersteller)
978-1-118-69673-6 (ISBN)

Infos

This textbook introduces readers to the modern light microscope. It can either be used alone or in conjunction with a practical course. The book begins with an account of how the light microscope works, and how to set up the instrument for optimum performance. The nature of light, and its influence on the function of the microscope, as well as practical advice on selecting the most suitable components, is explained to the reader. An account of the different types of currently-available light microscopy modes follows, together with a guide, borne out by the authors practical experience, on how to choose between transmitted or reflected light microscopes; wide-field, confocal, deconvolution or multi-photon microscopes. A description of specimen preparation is given, as well as advice upon cameras, image acquisition and post-processing of data.
* Gives a didactic introduction to the light microscope * Explanation complemented with simple experiments where appropriate * Simple explanation (from teaching the subject) enables a self-taught student to start from scratch to set up and acquire high quality images * Leads the student on, should they wish, to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility * Well-illustrated in colour, the illustrations are selected (from long teaching experience) to support text * Workable practical protocols * Includes a chapter summary to introduce learning objectives Part of the Royal Microscopical Society co-publication book program

Autor

JEREMY SANDERSON is a Bio-Imaging specialist at the Mammalian Genetics Unit, MRC Harwell Institute, in the UK. He is Chartered Scientist and a Fellow of the Royal Microscopical Society and has served both on the Executive Council and the LM Section Committee.

Inhalt

About the Author


Acknowledgements


Glossary and definitions


About the Companion Website


Notes


Introduction


Chapter 1 Our eyes and the microscope


Chapter 2 Light


Chapter 3 Basic microscope optics


Chapter 4 Microscope design


Chapter 5 Ergonomics


Chapter 6 Optical aberrations of the microscope


Chapter 7 The microscope objective


Chapter 8 Condensers & eyepieces


Chapter 9 Illumination in the microscope


Chapter 10 Diffraction and image formation in microscopy


Chapter 11 Contrast generation & enhancement


Chapter 12 Reflected-light microscopy


Chapter 13 Polarised-light microscopy: part 1 - theory


Chapter 14 Polarised-light microscopy: part 2 - applied


Chapter 15 Fluorescence microscopy


Chapter 16 Fluorophores & fluorescent proteins


Chapter 17 Optical sectioning & confocal microscopy


Chapter 18 Operating the confocal microscope


Chapter 19 Light-sheet microscopy


Chapter 20 Bleed-through & Spectral unmixing


Chapter 21 Deconvolution


Chapter 22 Multi-photon microscopy


Chapter 23 Total Internal Reflection Fluorescence microscopy


Chapter 24 FRAP and FRET


Chapter 25 Colocalisation


Chapter 26 Super-resolution fluorescence microscopy


Chapter 27 - Choosing a microscope platform and Core Imaging Facilities


Chapter 28 Biological Specimen Preparation


Chapter 29 Materials Specimen Preparation


Chapter 30 Recording the image: part 1 - theory


Chapter 31 Recording the image: part 2 - applied


Appendices


1. Buying and tendering for a light microscope


2. Trouble-shooting poor image quality


3. The Michel-Levy Interference chart


4. Cleaning and Maintenance


5. Microscope Manufacturer contacts


6. Brief historical notes


7. Timeline


Index

Produkt-Details

• Verlagsort: New York
• Sprache: englisch
• Maße: 150 x 250 mm
• Gewicht: 666 g
• Themenwelt: Naturwissenschaften ► Biologie
• Themenwelt: Naturwissenschaften ► Chemie
• Themenwelt: Technik ► Maschinenbau
• ISBN-10: 1-118-69673-5 / 1118696735
• ISBN-13: 978-1-118-69673-6 / 9781118696736
• Zustand: Neuware