Green Fluorescent Protein (eBook)

Properties, Applications and Protocols

Martin Chalfie, Steven Kain (Herausgeber)

eBook Download: PDF

2005 | 2. Auflage
464 Seiten
John Wiley & Sons (Verlag)
978-0-471-73948-7 (ISBN)

Infos

Since the discovery of the gene for green fluorescent protein
(GFP), derived from jellyfish, this protein that emits a green glow
has initiated a revolution in molecular biosciences. With this
tool, it is now possible to visualize nearly any protein of
interest in any cell or tissue of any species. Since the
publication of the first edition, there have been tremendously
significant technological advances, including development of new
mutant variants. Proteins are now available in yellow and blue, and
Novel Fluorescent Proteins (NFPs) have expanded their utility in
developing biosensors, biological markers, and other biological
applications.

This updated, expanded new edition places emphasis on the rise of
NFPs, including new chapters on NFP properties with detailed
protocols, applications of GFPs and NFPs in industry research, and
biosensors. This book provides a solid theoretical framework, along
with detailed, practical guidance on use of GFPs and NFPs with
discussion of potential pitfalls. The expert contributors provide
real examples in showing how to tailor GFP/NFP to specific systems,
maximize expression, and enhance detection.

Autor

MARTIN CHALFIE, PhD, is William R. Kenan Jr. Professor of Biological Sciences at Columbia University. He is world renowned for introducing the use of GFP as a biological marker. A member of the National Academy of Sciences, Dr. Chalfie serves on the editorial boards of Genome Biology and Molecular Biology of the Cell. STEVEN R. KAIN, PhD, manages the Applications Gene Expression Group for Agilent Technologies. While director of cell biology at Clontech Laboratories, he was responsible for commercializing the first products based on GFP and produced the popular EGFP variant. He has twelve years' experience with a variety of biotechnology firms in the San Francisco Bay Area.

Inhalt

First Edition Preface.

Preface.

Contributors.

1. Discovery of Green Fluorescent Protein (Osamu Shimomura).

2. Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems (J. Woodland Hastings and James G. Morin).

3. Biochemical and Physical Properties opf Green Fluorescent Protein (William W. Ward).

4. The Three-Dimensional Structure of Green Fluorescent Protein and Its Implications for Function and Design (George N. Phillips).

5. Molecular Biology and Mutation of Green Fluorescent Protein (David A. Zacharias and Roger Y. Tsien).

6. Discovery and Properties of GFP-Like Proteins from Nonbioluminescent Anthozoa (Konstantin A. Lukyanov, Dmitry M. Chudakov, Arkady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, and Sergey Lukyanov).

7. Evolution of Function and Color in GPF-Like Proteins (Mikhail V. Matz, Yulii A. Labas, and Juan Ugalde).

8. The Uses of Green Fluorescent Protein in Prokaryotes (Raphael H. Valdivia, Brendan P. Cormack, and Stanley Falkow).

9. The uses of Green Fluorescent Protein in Yeasts (Amy L. Hitchcock, Jason A. Kahana, and Pamela A. Silver).

10. Uses of GFP in Caenorhabditis Elegans (Oliver Hobert and Paula Loria).

11. Green Fluorescent Protein Applications in Drosophila (Tulle Hazelrigg and Jennifer H. Mansfield).

12.The Uses of Green Fluorescent Protein in Plants (Jim Haseloff and Kirby R. Siemering).

13. Uses of GFP in Transgenic Vertebrates (Sean Magason, Adam Amsterdam, Nancy Hopkins, and Shuo Lin).

14. The Uses of Green Fluorescent Protein in Mammalian Cells (Theresa H. Ward and Jennifer Lippincott-Schwartz).

15. Practical Considerations for Use of Reef Coral Fluorescent Proteins in Mammalian Cells: Applications in Fluorescent Microscopy and Flow Cytometry (Yu Fang, Olivier D??ry, Michael Haugwitz, Pierre Turpin, and Steven R. Kain).

16. Pharmaceutical Applications of GFP and RCFP (Nicola Bevan and Stephen Rees).

17. Reassembled GFP: Detecting Protein-Protein Interactions and Protein Expression Patterns (Thomas J. Magliery and Lynne Regan).

Methods and Protocols (Steven R. Kain).

Index.

Rezensionen

"...a magnificent text that will immensely benefit students and
scientists from many biomedical areas." (Annals of Biomedical
Engineering, August 2006)

"Any researcher, graduate student or educator with an interest
in using GFP should read this book. It will become the 'bible for
GFP' users." (The Quarterly Review of Biology, June
2006)

Produkt-Details

• Erscheint lt. Verlag: 13.12.2005
• Reihe/Serie: Methods of Biochemical Analysis
• Reihe/Serie: Methods of Biochemical Analysis
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• Themenwelt: Naturwissenschaften ► Chemie ► Organische Chemie
• Themenwelt: Technik
• Schlagworte: Biochemie • Biochemie u. Chemische Biologie • Biochemistry (Chemical Biology) • Biotechnologie i. d. Biowissenschaften • Biotechnology • Biowissenschaften • Chemie • Chemistry • Life Sciences • Pharmaceutical & Medicinal Chemistry • Pharmazeutische u. Medizinische Chemie
• ISBN-10: 0-471-73948-0 / 0471739480
• ISBN-13: 978-0-471-73948-7 / 9780471739487