Mass Spectrometry of Protein Interactions (eBook)

Kevin Downard (Herausgeber)

eBook Download: PDF

2007 | 1. Auflage
152 Seiten
John Wiley & Sons (Verlag)
978-0-470-14632-3 (ISBN)

Infos

The authoritative guide to analyzing protein interactions by mass
spectrometry

Mass spectrometry (MS) is playing an increasingly important role in
the study of protein interactions. Mass Spectrometry of Protein
Interactionspresents timely and definitive discussions of the
diverse range of approaches for studying protein interactions by
mass spectrometry with an extensive set of references to the
primary literature. Each chapter is written by authors or teams of
authors who are international authorities in their fields. This
leading reference text:

* Discusses the direct detection of protein interactions through
electrospray ionization (ESI-MS); ion mobility analysis; and
matrix-assisted laser desorption/ionization (MALDI-MS)

* Covers the indirect analysis of protein interactions through
hydrogen-deuterium exchange (HX-MS); limited proteolysis;
cross-linking; and radial probe (RP-MS)

* Guides researchers in the use of mass spectrometry in structural
biology, biochemistry, and protein science to map and define the
huge number and diversity of protein interactions

* Reviews the latest discoveries and applications and addresses new
and ongoing challenges

This is a comprehensive reference for researchers in academia and
industry engaged in studies of protein interactions and an
excellent text for graduate and postgraduate students.

Autor

Kevin M. Downard, PhD, is an Associate Professor in the School of Molecular and Microbial Biosciences at the University of Sydney in Australia. He has twenty years of research experience in mass spectrometry, with the past fifteen devoted to protein analysis.

Inhalt

Preface.

Contributors.

Chapter 1. Direct Characterization of Protein Complexes by
Electrospray Ionization Mass Spectrometry and Ion Mobility Analysis
(Joseph A. Loo and Catherine S. Kaddis).

1.1 Introduction.

1.2 Critical Aspects of the Experimental Procedure.

1.3 Solution Phase Equilibria and Gas Phase Dissociation.

1.4 Conclusions.

Chapter 2. Softly, Softly-Detection of Protein Complexes by
Matrix-Assisted Laser Desorption Ionisation Mass Spectrometry
(Kevin M. Downard).

2.1 Introduction.

2.2 First Glimpses and the First-Shot Phenomenon.

2.3 Matrix and Solution Criteria to Preserve Protein
Complexes.

2.4 Laser Fluence, Wavelength and Ion Extraction.

2.5 Preservation of Protein Complexes on Conventional Maldi
Targets.

2.6 Affinity Targets and Surfaces Coupled To Maldi.

2.7 Conclusions.

Chapter 3. Probing Protein Interactions Using
Hydrogen-Deuterium Exchange Mass Spectrometry (David D. Weis, Suma
Kaveti, Yan Wu and John R. Engen).

3.1 Introduction.

3.2 Hydrogen Exchange Background.

3.3 General HX-MS Method.

3.4 Interactions of Proteins.

3.5 Examples.

3.6 Conclusions.

Chapter 4. Limited Proteolysis Mass Spectrometry of Protein
Complexes (Maria Monti and Piero Pucci).

4.1 Introduction.

4.2 Limited Proteolysis Analysis.

4.3 Experimental Design.

4.4 Probing Protein-Protein Interactions.

4.5 Probing Protein-Nucleic Acid Interactions.

4.6 Protein-Ligand Interactions.

4.7 Probing Amyloid Fibril Core.

4.8 Conlusions.

Chapter 5. Chemical Cross-Linking and Mass Spectrometry for
Investigation of Protein-Protein Interactions (Andrea
Sinz).

Introduction.

5.1 Cross-Linking Strategies.

5.2 Functional Groups of Cross-Linking Reagents.

5.3 Mass Spectrometric Analysis of Cross-Linked Products.

5.4 Identification of Cross-Linked Produc.

5.5 Computer Software for Data Analysis.

5.6 Conclusions and Perspectives.

Chapter 6. Genesis & Application of Radical Probe Mass
Spectrometry (RP-MS) to Study Protein Interactions (Simin D.
Maleknia and Kevin M. Downard).

6.1 Genesis of Radical Probe Mass Spectrometry.

6.2 The Reactive Residue Side Chains.

6.3 Conditions Important to Radical Probe Mass Spectrometry
Experiments.

6.4 Generation of Radicals on Millisecond Timescales.

6.5 Applications of RP-MS to Studies of Protein
Interactions.

6.6 Onset of Oxidative Damage and Its Implications for Protein
Interactions.

6.7 Application of Radical Oxidation to Study Protein
Assemblies.

6.8 Modeling Protein Complexes with Data from RP-MS
Experiments.

6.9 Conclusions.

Index.

Produkt-Details

• Erscheint lt. Verlag: 28.6.2008
• Reihe/Serie: Wiley-Interscience Series on Mass Spectrometry
• Reihe/Serie: Wiley-Interscience Series on Mass Spectrometry
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Chemie ► Analytische Chemie
• Themenwelt: Naturwissenschaften ► Physik / Astronomie
• Themenwelt: Technik
• Schlagworte: Biowissenschaften • Cell & Molecular Biology • Chemie • Chemistry • Life Sciences • Massenspektrometrie • Massenspektroskopie • Mass Spectrometry • Protein • Zell- u. Molekularbiologie
• ISBN-10: 0-470-14632-X / 047014632X
• ISBN-13: 978-0-470-14632-3 / 9780470146323