Genomics (eBook)

Essential Methods

Mike Starkey, Ramnath Elaswarapu (Herausgeber)

eBook Download: PDF

2010 | 1. Auflage
360 Seiten
John Wiley & Sons (Verlag)
978-0-470-71162-0 (ISBN)

Infos

Genomics research has made significant advances in recent years. In
this book, a team of internationally-renowned researchers share the
most up-to-date information in a field that has in recent years
switched emphasis from gene identification to functional genomics
and the characterization of genes and gene products. This volume
approaches this complex subject with a broad perspective to supply
the reader with a vital overview of genomics and its derivative
fields, with a focus on pivotal issues such as data analysis.
Expansive and current, this book is a comprehensive
research guide that describes both the key new techniques and more
established methods. Every chapter discusses the merits and
limitations of the various approaches and then provides selected
tried-and-tested protocols, as well as a plethora of good practical
advice for immediate use at the bench.

Key features:

* Provides a broad introduction to current practices and
techniques for lab-based research in genomics

* Explains clearly and precisely how to carry out selected
techniques in addition to background information on the various
approaches

* Chapters are written by a leading international authorities in
the field and cover both well-known and new, tried and tested,
methods for working in genomics

* Includes troubleshooting guide and reviews of alternative
techniques

* An essential laboratory manual for students and researchers at
all levels

Autor

Dr Mike Starkey, Animal Health Trust, Newmarket, Suffolk, UK. Dr Ramnath Elaswarapu, Oxford Gene Technology Ltd, Yarnton, Oxford, UK.

Inhalt

List of Contributors.

Preface.

1 High-Resolution Analysis of Genomic Copy Number Changes
(Mario Hermsen, Jordy Coffa, Bauke Ylstra, Gerrit Meijer, Hans
Morreau, Ronald van Eijk, Jan Oosting and Tom van Wezel).

1.1 Introduction.

1.2 Methods and Approaches.

1.3 Troubleshooting.

References.

2 Identification of Polymorphic Markers for Genetic
Mapping (Daniel C. Koboldt and Raymond D. Miller).

2.1 Introduction.

2.2 Methods and Approaches.

2.3 Troubleshooting.

References.

3 Genotyping and LOH Analysis on Archival Tissue Using SNP
Arrays (Ronald van Eijk, Anneke Middeldorp, Esther H. Lips,
Marjo van Puijenbroek, Hans Morreau, Jan Oosting and Tom van
Wezel).

3.1 Introduction.

3.2 Methods and Approaches.

3.3 Troubleshooting.

References.

4 Genetic Mapping of Complex Traits (Nancy L.
Saccone).

4.1 Introduction.

4.2 Methods and Approaches.

4.3 Troubleshooting.

References.

5 RNA Amplification Strategies: Toward Single-Cell
Sensitivity (Natalie Stickle, Norman N. Iscove, Carl
Virtanen, Mary Barbara, Carolyn Modi, Ari Baratz, Toni Di
Berardino, Ellen Greenblatt, Ted Brown and Neil
Winegarden).

5.1 Introduction.

5.2 Methods and Approaches.

5.3 Troubleshooting.

References.

6 Real-Time Quantitative RT-PCR for mRNA Profiling
(Stephen A. Bustin and Tania Nolan).

6.1 Introduction.

6.2 Methods and Approaches.

6.3 Troubleshooting.

References.

7 Gene Expression in Mammalian Cells (Félix
Recillas-Targa, Georgina Guerrero, Martín Escamilla-del-Arenal
and Héctor Rincón-Arano).

7.1 Introduction.

7.2 Methods and Approaches.

7.3 Troubleshooting.

Acknowledgments.

References.

8 Using Yeast Two-Hybrid Methods to Investigate Large Numbers
of Binary Protein Interactions (Panagoula Charalabous,
Jonathan Woodsmith and Christopher M. Sanderson).

8.1 Introduction.

8.2 Methods and Approaches.

8.3 Troubleshooting.

References.

9 Prediction of Protein Function (Hon Nian
Chua).

9.1 Introduction.

9.2 Methods and Approaches.

9.3 Troubleshooting.

References.

10 Elucidating Gene Function through Use of Genetically
Engineered Mice (Mary P. Heyer, Cátia Feliciano,
João Peca and Guoping Feng).

10.1 Introduction.

10.2 Methods and Approaches.

10.3 Troubleshooting.

References.

11 Delivery Systems for Gene Transfer (Charlotte
Lawson and Louise Collins).

11.1 Introduction.

11.2 Methods and Approaches.

11.3 Troubleshooting.

References.

12 Gene Therapy Strategies: Constructing an AAV Trojan
Horse (M. Ian Phillips, Edilamar M. de Oliveira, Leping
Shen, Yao Liang Tang, and Keping Qian).

12.1 Introduction.

12.2 Methods and Approaches.

12.3 Troubleshooting.

References.

13 An Introduction to Proteomics Technologies for the
Genomics Scientist (David B. Friedman).

13.1 Introduction.

13.2 Methods and Approaches.

13.3 Troubleshooting.

References.

Index.

Produkt-Details

• Erscheint lt. Verlag: 19.10.2010
• Reihe/Serie: Essential Methods
• Reihe/Serie: Essential Methods
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• Themenwelt: Technik
• Schlagworte: Biowissenschaften • Genomforschung u. Proteomik • genomics • Genomics & Proteomics • Life Sciences
• ISBN-10: 0-470-71162-0 / 0470711620
• ISBN-13: 978-0-470-71162-0 / 9780470711620