Engineering Complex Phenotypes in Industrial Strains (eBook)

Ranjan Patnaik (Autor)

eBook Download: EPUB

2012 | 1. Auflage
280 Seiten
John Wiley & Sons (Verlag)
978-1-118-43304-1 (ISBN)

Infos

This book highlights current trends and developments in the area of
engineering strains. The book details the current and future tools
used in the production of bulk chemicals and biofuels from
renewable biomass using green technologies. Complex phenotypes are
traits in a microbe that requires multiple genetic changes to be
modulated simultaneously in the microorganism's DNA. Knowing
what those genetic changes are for a given trait and how to make
those changes in the most efficient way forms the motivation behind
writing this book. This book explains the newer tools to develop
and enable engineered strains at time scales much faster that the
natural evolution process so that we can increase a cells'
production of a certain substance, increase process productivity,
and extend metabolic capability. This book provides a one stop
platform of reference for practicing researchers in the field of
industrial biotechnology. This book also explains that the
commercial success of a process that uses microbial catalysts over
platforms that use chemical catalysts and fossil fuels depends on
the time it takes to engineer these microbes to perform the desired
reaction under harsh manufacturing conditions and at rates that
meets the criteria for economic feasibility.

Autor

RANJAN PATNAIK, PhD, is a chemical engineer by profession with 17 years of practical experience in the field of industrial biotechnology that includes small biotech start-ups and large enterprises. His research interests are in the field of metabolic engineering, fermentation optimization, process integration and R&D technology management. He currently heads the biofuels research group at DuPont Knowledge Center (DKC), Hyderabad, India. Prior to his current role, he had a variety of responsibilities within the biochemical sciences & engineering division of CR&D, E. I. du Pont de Nemours and Company, Wilmington, DE, USA.

Inhalt

Foreword vii

John Pierce

Preface ix

Contributors xi

1 Classical Strain Improvement 1

Nathan Crook and Hal S. Alper

2 Tracer-Based Analysis of Metabolic Flux Networks 35

Michael Dauner

3 Integration of "Omics" Data with Genome-Scale
Metabolic Models 77

Stephen Van Dien, Priti Pharkya, and Robin Osterhout

4 Strain Improvement via Evolutionary Engineering 111

Byoungjin Kim, Jing Du, and Huimin Zhao

5 Rapid Fermentation Process Development and Optimization
133

Jun Sun and Lawrence Chew

6 The Clavulanic Acid Strain Improvement Program at DSM
Anti-Infectives 169

Bert Koekman and Marcus Hans

7 Metabolic Engineering of Recombinant E. coli for the
Production of 3-Hydroxypropionate 185

Tanya Warnecke Lipscomb, Matthew L. Lipscomb, Ryan T. Gill, and
Michael D. Lynch

8 Complex System Engineering: A Case Study for an Unsequenced
Microalga 201

Michael T. Guarnieri, Lieve M.L. Laurens, Eric P. Knoshaug,
Yat-Chen Chou, Bryon S. Donohoe, and Philip T. Pienkos

9 Meiotic Recombination-Based Genome Shuffling of Saccharomyces
Cerevisiae and Schefferomyces Stiptis for Increased Inhibitor
Tolerance to Lignocellulosic Substrate Toxicity 233

Dominic Pinel and Vincent J.J. Martin

Index 251

Rezensionen

"This book should be valuable to engineers, biochemists,
and students who seek to understand the science and practice
of engineering biocatalysts for industrial
applications." (Chemical Engineering
Progress, 1 January 2013)

Produkt-Details

• Erscheint lt. Verlag: 19.9.2012
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie
• Themenwelt: Naturwissenschaften ► Chemie ► Technische Chemie
• Themenwelt: Technik ► Umwelttechnik / Biotechnologie
• Schlagworte: Biochemie u. Chemische Biologie • Biochemistry (Chemical Biology) • Biowissenschaften • Cell & Molecular Biology • chemical engineering • Chemie • Chemische Verfahrenstechnik • Chemistry • Life Sciences • Zell- u. Molekularbiologie
• ISBN-10: 1-118-43304-1 / 1118433041
• ISBN-13: 978-1-118-43304-1 / 9781118433041