Evolutionary Methods in Biotechnology (eBook)

Clever Tricks for Directed Evolution

Susanne Brakmann, Andreas Schwienhorst (Herausgeber)

eBook Download: PDF

2006 | 1. Auflage
XIII, 214 Seiten
Wiley-VCH (Verlag)
978-3-527-60462-3 (ISBN)

Infos

Miniturization and high throughput assay technology have brought the power of molecular evolution to the bioscience laboratory. Applied wisely, the evolutionary approach can quickly yield the desired result even where other methods have failed.
From library generation by random or directed mutagenesis to screening and selection techniques -- the crucial steps for successful evolutionary biotechnology are described in detail in this practical guide that also includes valuable troubleshooting hints on frequently encountered problems.
Modern methods for the surface display of peptides and proteins, selective enrichment of nucleic acid aptamers and high-throughput screening of industrial biocatalysts are explained, and computer-based methods for in silico protein and RNA engineering are described as an alternative to in vitro approaches. A special section covers the patenting regulations with regard to biotechnological innovations derived from directed evolution.
As an added bonus, a CD-ROM is included that contains software tools for library design, selection of mutagenesis positions, and various predictive algorithms.
In short, this practice oriented handbook is an indispensable tool for every scientist working in this interdisciplinary research area.

Wie erstellt und durchsucht man kombinatorische Enzymbibliotheken? Dieser Band stellt Ihnen erstmals geprüfte, ausführliche Protokolle zur Verfügung. Die Autoren der Beiträge, Experten mit eigener Erfahrung in der evolutionären Biotechnologie, kommentieren die Arbeitsanleitungen mit Hinweisen zu häufigen Fehlern und deren Beseitigung. Unentbehrlich für jeden, der auf diesem interdisziplinären Gebiet arbeitet.

Autor

Susanne Brakmann is head of the junior research group "Applied Molecular Evolution" at the University of Leipzig (Germany) and a Member of the Biotechnological-Biomedical Center of Leipzig. She studied Chemistry at the Technical University of Braunschweig where she received her diploma in 1988, moving afterwards to the University of Karlsruhe to work on her thesis under the supervision of Reinhold Tacke (Ph. D. 1991). She was postdoctoral fellow at the Max-Planck-Institute for Biophysical Chemistry in Göttingen where she worked with Manfred Eigen before she moved to Leipzig in 2001. She is interested in directed evolution as a tool for understanding and optimizing enzyme functions, focusing on nucleic acid polymerases and their biotechnological applications. Andreas Schwienhorst is a group leader at the Institute of Microbiology and Genetics at the University of Göttingen (Germany). He was born in Warendorf and did his studies of Biochemistry and Prehistory in Münster and Göttingen. His thesis was carried out at the Max-Planck-Institute for Biophysical Chemistry under the supervision of Manfred Eigen. He was a visiting scientist at the Salk Institute, La Jolla and the Los Alamos National Laboratory (USA), then took a position as a group leader at the Institute for Molecular Biotechnology before moving to the University of Göttingen in 1997. In 1998 he received the Biofuture Award. Currently, he is interested in the discovery of novel targets for drug intervention as well as in methods of molecular evolution and their applications in drug discovery and catalysis.

Inhalt

Introduction
Generation of Mutant Libraries Using Random Mutagenesis
DNA Shuffling
DNA Recombination Using StEP
FACS Screening of Combinatorial Peptide and Protein Libraries
Selection of Phage-displayed Enzymes
Selection of Aptamers
Methods for Selecting Catalytic Nucleic Acids
High-Throughput Screening of Enantioselective Industrial Biocatalysts
Computer-assisted Design of Doped Libraries
Directed in silico Mutagenesis
RNA Folding in silico
Patenting in Evolutionary Biotechnology
Programs on CD: How to identify positions suited for mutagenesis; Folding aspects; databases; structure-based design; and more
Glossary and Index
Introduction
Generation of Mutant Libraries Using Random Mutagenesis
DNA Shuffling
DNA Recombination Using StEP
FACS Screening of Combinatorial Peptide and Protein Libraries
Selection of Phage-displayed Enzymes
Selection of Aptamers
Methods for Selecting Catalytic Nucleic Acids
High-Throughput Screening of Enantioselective Industrial Biocatalysts
Computer-assisted Design of Doped Libraries
Directed in silico Mutagenesis
RNA Folding in silico
Patenting in Evolutionary Biotechnology
Programs on CD: How to identify positions suited for mutagenesis; Folding aspects; databases; structure-based design; and more
Glossary and Index

Rezensionen

"From library generation by random or directed mutagenesis to screening and selection techniques, the crucial steps for succesful evolutionary biotechnology are described in detail in this practical guide, that also includes valuable troubleshooting hints on frequently encountered problems." Process

"... contains valuable information." Molecular Biotechnology

"... is truly a highly practical handbook, a "must have" for any laboratory practicing directed evolution of proteins, and a methodological "cookbook" of high quality." Angewandte Chemie I.E.

"...this volume can be recommended to novices desirous to get started with evolutionary methods as well as experienced scientists of the field." Carbohydrate Polymers

"... a useful snapshot of the state of the art of directed evolution methods for academia and industrial working groups." The Quarterly Review of Biology

"... a terrific collection of useful tricks and protocols that can be wholeheartedly recommended." ASM News

"Das Buch ist ... ein unentbehrlicher Helfer für jeden, der auf diesem interdisziplinären Gebiet arbeitet."
PROCESS, PharmaTEC

"Das vorliegende Buch ist eine sehr erfreuliche, sorgfältig editierte und redigierte Neuerscheinung..."
Nachrichten aus der Chemie

"... ist gleichermaßen ein überaus praktisches Handbuch, ein "must have" für Wissenschaftler, die sich mit der gerichteten Evolution von Proteinen beschäftigen, und ein methodenorientiertes "Kochbuch" von höchster Qualität."
Angewandte Chemie und Angewandte I.E.

"...für all jene, die bereits auf dem Gebiet Fuß gefaßt haben, eine Bereicherung und interessante Anregung...gibt Neulingen und Erfahrenen gleichermaßen einen gelungenen und vielseitigen Überblick zum methodischen "state of the art" biotechnologischer Evolutionsansätze."
BIOspektrum

"Dieses Buch ist nicht dazu da, sich mit ihm in ein Cafe zu setzen und gemütlich in der Welt der Biotechnologie zu schmökern, sondern um damit im Labor zu stehen und damit zu arbeiten."
Laborjournal

Produkt-Details

• Erscheint lt. Verlag: 6.3.2006
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Chemie ► Organische Chemie
• Themenwelt: Technik
• Schlagworte: Biochemie u. Chemische Biologie • Biochemistry (Chemical Biology) • biomedical engineering • Biomedizintechnik • Biotechnologie • Biotechnologie i. d. Biowissenschaften • Biotechnology • Biowissenschaften • Chemie • Chemistry • Genetics • Genetik • Life Sciences • Organic Chemistry • Organische Chemie • Protein Engineering • Protein-Engineering
• ISBN-10: 3-527-60462-6 / 3527604626
• ISBN-13: 978-3-527-60462-3 / 9783527604623