Biophysics and the Challenges of Emerging Threats (eBook)

Joseph Puglisi (Herausgeber)

eBook Download: PDF

2009 | 2009
VII, 179 Seiten
Springer Netherland (Verlag)
978-90-481-2368-1 (ISBN)

Infos

Proceedings of the NATO Advanced Study Institute on Biophysics and the Challenges of Emerging Threats
Erice, Sicily, Italy
19-30 June 2007

---

Single-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems [1–3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding [4–6], enzyme kinetics [7, 8], and even protein biosynthesis [1, 9, 10]. In particular, immobilization-based fluorescence te- niques such as total internal reflection fluorescence microscopy (TIRF-M) have recently allowed for the observation of multiple events on the millis- onds to seconds timescale [11–13]. Single-molecule fluorescence methods are challenged by the instability of single fluorophores. The organic fluorophores commonly employed in single-molecule studies of biological systems display fast photobleaching, intensity fluctuations on the millisecond timescale (blinking), or both. These phenomena limit observation time and complicate the interpretation of fl- rescence fluctuations [14, 15]. Molecular oxygen (O) modulates dye stability. Triplet O efficiently 2 2 quenches dye triplet states responsible for blinking. This results in the for- tion of singlet oxygen [16–18]. Singlet O reacts efficiently with organic dyes, 2 amino acids, and nucleobases [19, 20]. Oxidized dyes are no longer fluor- cent; oxidative damage impairs the folding and function of biomolecules. In the presence of saturating dissolved O, blinking of fluorescent dyes is sup- 2 pressed, but oxidative damage to dyes and biomolecules is rapid. Enzymatic O -scavenging systems are commonly employed to ameliorate dye instability. 2 Small molecules are often employed to suppress blinking at low O levels.

Inhalt

Preface.- List of Contributors.- A Simple Model for Protein Folding; E.R. Henry, W.A. Eaton.- Complementarity of Hydrophobic/Hydrophilic Properties in Protein Ligand Complexes: A New Tool to Improve Docking Results; T.V. Pyrkov et al.- Structures of Cvnh Family Lectins; A.M. Gronenborn.- Biohysical Approaches to Study DNA Base Flipping; S. Klimasauskas et al.- The Diversity of Nuclear Magnetic Resonance Spectroscopy; C.W. Liu.- Improved Dye Stability in Single-Molecule Fluorescence Experiments; C.E. Aitken et al.- The Evaluation of Isotope Editing and Filtering for Protein-Ligand Interaction Elucidation by NMR; I.M. Robertson et al.- Ribosome: an Ancient Cellular Nano-Machine for Genetic Code Translation; A. Yonath.- Course Abstracts and Posters.- Author Index.-

Produkt-Details

• Reihe/Serie: NATO Science for Peace and Security Series
• Verlagsort: Dordrecht
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
• Themenwelt: Technik ► Umwelttechnik / Biotechnologie
• Schlagworte: Base • biochemistry • Biological Dynamics • Biomolecular NMR • Biophysics • Biotechnology • DNA • fluorescence • Infectious Agents • Magnetic Resonance • NATO • Peace • Physics • Protein and Nucleic Acid Structure • Science • security • Single-Molecule Biophysics • Sub-Series B • Translation
• ISBN-10: 90-481-2368-2 / 9048123682
• ISBN-13: 978-90-481-2368-1 / 9789048123681