Dry Chemistry (eBook)

Analysis with Carrier-bound Reagents

O. Sonntag (Autor)

eBook Download: EPUB

1993 | 1. Auflage
640 Seiten
Elsevier Science (Verlag)
978-0-08-085894-4 (ISBN)

Infos

Dry chemistry has been accepted as an important technology in medical laboratories for many years. Many evaluations of this technology have been undertaken by reputable clinical laboratories, the results of which were excellent when compared with conventional wet chemistry analysis.

This book contains a detailed overview of the current knowledge in the field of dry chemistry both in the physicians' office laboratories and large medical laboratories. The results from many evaluation studies are presented, as is data from interference studies which complete the descriptions of many dry chemistry methods.

A detailed description of various commercially available dry chemistry systems such as Ektachem, Reflotron, Seralyzer, Cobas Ready, Drichem, Opus and Stratus are also included.

This book effectively describes the current state-of-the-art technology and knowledge and succeeds in filling the gap in information in this important field of clinical chemistry science.

Originally published as 'Trockenchemie' by Georg Thieme Verlag,
Stuttgart, Dr. Sonntag has taken the opportunity of this translation to completely revise and update the contents of his book.

Inhalt

Cover 1
TOC$Contents 12
Preface 8
Preface of the German edition 10
CH$Chapter 1. Introduction to dry chemistry 18
What is dry chemistry? 19
History of development 20
References 22
CH$Chapter 2. Theory of reflection spectroscopy 24
Terminology 24
Fundamentals of reflection spectroscopy 26
References 31
CH$Chapter 3. Instrument systems 36
General pointers on the use of the data sheets 36
CH$Chapter 4. Cobas Ready 38
Description of the reagent strips 40
Reflectometric measurement system 42
Reflectance measurement 43
Calculation 44
Calibration 45
Alanine aminotransferase (ALT) 45
Albumin 46
Alkaline phosphatase (ALP) 47
a-Amylase 48
Aspartate aminotransferase (AST) 50
Bilirubin, total 51
Calcium 52
Cholesterol 53
HDL cholesterol 54
Creatine kinase (CK) 56
Creatinine 58
Glucose 59
.-Glutamyl transferase (.-GT) 60
Lactate dehydrogenase (LDH) 61
Protein, total 62
Triglycerides 63
Urea 64
Uric acid 65
References 67
CH$Chapter 5. Drichem 1000 68
Glucose 68
Urea 70
References 73
CH$Chapter 6. Ektachem 74
Description of the slides 74
Reagent carrier for electrolyte assays 78
Potentiometric measurements 78
Description of the Ektachem DT-60 II 80
DTE module 82
DTSC module for determining the enzyme activity 83
Ektachem 700 86
Acid phosphatase (AcP) 96
Alanine aminotransferase (ALT) or glutamate pyruvate transaminase (GPT) 101
Albumin 105
Alkaline phosphatase (ALP) 109
Ammonia 112
Amylase 116
Aspartate aminotransferase (AST) or glutamate oxalacetate transaminase (GOT) 120
Bilirubin, total 124
Bilirubin, unconjugated and conjugated 130
Calcium 139
Carbon dioxide 144
Carbon dioxide, enzymatic 149
Chloride 154
Cholesterol 158
HDL cholesterol 163
Cholinesterase (CHE) 166
C-reactive protein (CRP) 172
Creatine kinase (CK) 174
Creatine kinase MB (CK-MB) 178
Creatinine (single-slide method) 181
Creatinine (two-slide method) 187
Ethanol 193
Glucose 198
.-Glutamyl transferase (.-GT) 207
Haemoglobin (only on DT-systems) 210
Iron 214
Lactate 217
Lactate dehydrogenase (LDH) 219
Leucine amino peptidase (LAP) (preliminary) 223
Lipase 226
Lithium 230
Magnesium 236
Phosphate 240
Potassium 244
Protein 248
Protein in CSF (cerebrospinal fluid) 251
Salicylate 256
Sodium 259
Theophylline 263
Total iron-binding capacity 267
Triglycerides 269
Urea 273
Uric acid 281
Remarks 286
References 288
CH$Chapter 7. Opus 338
Analyzer description 339
Optical measurement 340
Continuous access 340
Opus assays 341
References 345
CH$Chapter 8. Reflotron 348
Air displacement pipette 350
Description of the reagent carrier 352
Reflection spectrometer 356
Data management 357
Calculation 357
Alanine aminotransferase (ALT) or glutamate pyruvate transaminase (GPT) 359
a-Amylase 365
Amylase, pancreatic 370
Aspartate aminotransferase (AST or glutamate oxalacetate transaminase (GOT) 374
Bilirubin 379
Cholesterol 383
HDL cholesterol 389
Creatine kinase (CK) 394
Creatinine 398
Glucose 403
.-Glutamyl transferase (.-GT) 408
Haemoglobin 415
Potassium 420
Triglycerides 424
Urea 429
Uric acid 434
Remarks 438
References 439
CH$Chapter 9. Seralyzer 452
Pipette system 453
Description of the reagent carriers 454
Reflection spectrometer 456
Measurement module 458
Calculation 458
Calibration 459
Performance of a measurement 459
Alanine aminotransferase (ALT) or glutamate pyruvate transaminase (GPT) 462
Aspartate aminotransferase (AST) or glutamate oxalacetate transaminase (GOT) 465
Bilirubin 468
Carbamazepine 475
Cholesterol 478
Creatine kinase (CK) 484
Creatinine 489
Digoxin 495
Glucose I 499
Glucose II (Hexokinase) 506
Haemoglobin 510
Lactate dehydrogenase (LDH) 512
Phenobarbital 518
Phenytoin 522
Potassium 526
Theophylline 529
Triglycerides 536
Urea 538
Uric acid 545
Remarks 550
References 551
CH$Chapter 10. Stratus 568
Stratus analyzer 569
Description of the method 572
Structure of the test tab 572
Fluorimetry 574
Calibration 575
Amikacin 576
Carbamazepine 577
Chorionic gonadotropin (human chorionic gonadotropin) 578
Cortisol 579
Creatine kinase MB (CK-MB) 581
Digitoxin 582
Digoxin 583
Ferritin 584
Follicle stimulating hormone (hFSH) 585
Free thyroxine (free T4) 586
Gentamicin 587
Human thyroid stimulating hormone (hTSH) 589
Immunoglobulin E (IgE) 590
Lidocaine 591
Luteinising hormone (hLH) 592
Phenobarbital 593
Phenytoin 594
Primidone 595
Quinidine 597
Theophylline 598
Thyroid uptake (TU) 599
Tobramycin 600
Total thyroxine (T4) 601
Total triiodothyronine (T3) 602
Remarks 603
References 604
CH$Chapter 11. Dry chemistry as used in the physician's office laboratory 614
References 615
CH$Chapter 12. Summary 618
Volume of the sample 618
Sample material 626
Chromogens in the sample material 626
Interferences 627
Dwelling times up to the performance of analysis 627
Calibration 628
Quality control 629
Storage life and stability 630
Costs 631
Outlook 631
Glossary 634
IDX$Index 636

Produkt-Details

• Erscheint lt. Verlag: 14.10.1993
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Laboratoriumsmedizin
• Themenwelt: Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Chemie ► Analytische Chemie
• Themenwelt: Technik ► Medizintechnik
• ISBN-10: 0-08-085894-5 / 0080858945
• ISBN-13: 978-0-08-085894-4 / 9780080858944