Confocal Microscopy (eBook)

P. Michael Conn (Herausgeber)

eBook Download: PDF

1999 | 1. Auflage
663 Seiten
Elsevier Science (Verlag)
978-0-08-049665-8 (ISBN)

Infos

This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Inhalt

Cover 1
Table of Contents 6
Contributors to Volume307 10
Preface 14
Volumes in Series 16
Section I: Theory and Practical Considerations 34
Chapter 1. Theoretical Basis of Confocal Microscopy 36
Chapter 2. Practical Considerations in Acquiring Biological Signals from Confocal Microscope 53
Section II: General Techniques 60
Chapter 3. Equipment for Mass Storage and Processing of Data 62
Chapter 4. Antifading Agents for Confocal Fluorescence Microscopy 88
Chapter 5. Mounting Techniques for Confocal Microscopy 112
Chapter 6. Preparation of Whole Mounts and Thick Sections for Confocal Microscopy 117
Chapter 7. Cytotomography 141
Chapter 8. Use of Confocal Microscopy to Investigate Cell Structure and Function 152
Chapter 9. Combining Laser Scanning Confocal Microscopy and Electron Microscopy 168
Chapter 10. Construction of Line-Scan Confocal Microscope for Physiological Recording 185
Section III: Measurement of Subcellular Relations and Volume Determinations 204
Chapter 11. Three-Dimensional Visualization of Cytoskeleton by Confocal Laser Scanning Microscopy 206
Chapter 12. Chromosome Spread for Confocal Microscopy 223
Chapter 13. Visualization of Nuclear Pore Complex and Its Distribution by Confocal Laser Scanning Microscopy 240
Chapter 14. Measurement of Tissue Thickness Using Confocal Microscopy 263
Chapter 15. Measurements of Vascular Remodeling by Confocal Microscopy 279
Chapter 16. Cell Volume Measurements by Fluorescence Confocal Microscopy: Theoretical and Practical Aspects 307
Chapter 17. Volume Measurements in Confocal Microscopy 329
Section IV: Imaging of Processes 350
Chapter 18. Quantitation of Phagocytosis by Confocal Microscopy 352
Chapter 19. Measurement of Secretion in Confocal Microscopy 361
Chapter 20. Receptor–Ligand Internalization 373
Chapter 21. Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy 384
Chapter 22. Localization of Proteases and Peptide Receptors by Confocal Microscopy 401
Chapter 23. Observation of Microcirculatory Kinetics by Real-Time Confocal Laser Scanning Microscopy 427
Section V: Imaging of Ions 456
Chapter 24. Confocal Imaging Analysis of Intracellular Ions in Mixed Cellular Systems or in Situ Using Two Types of Confocal Microscopic Systems 458
Chapter 25. Confocal Ca2+ Imaging of Organelles, Cells, Tissues, and Organs 474
Chapter 26. Using Confocal Microscopy and Fluorescent Indicator, 6-Methoxy-N-ethylquinolinium Iodide, to Measure Changes in Intracellular Chloride 502
Section VI: Imaging of Specialized Tissues 516
Chapter 27. Measurement of Mineral Gain and Loss on Dental Enamel and Dentin Using Confocal Microscopy 518
Chapter 28. Characterization of Ocular Cellular and Extracellular Structures Using Confocal Microscopy and Computerized Three-Dimensional Reconstruction 529
Chapter 29. Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues: Functional Metabolic Imaging of Human Skin in Vivo 546
Chapter 30. Video-Rate, Scanning Slit Confocal Microscopy of Living Human Cornea in Vivo: Three-Dimensional Confocal Microscopy of the Eye 569
Chapter 31. In Vivo Imaging of Mammalian CNS Neurons with ICON 596
Section VII: Imaging Viruses and Fungi 604
Chapter 32. Identification of Viral Infection by Confocal Microscopy 606
Chapter 33. Using Confocal Microscopy to Study Virus Binding and Entry into Cells 624
Chapter 34. Fluorescent Labels, Confocal Microscopy, and Quantitative Image Analysis in Study of Fungal Biology 640
Author Index 658
Subject Index 680

Produkt-Details

• Erscheint lt. Verlag: 7.9.1999
• Mitarbeit: Chef-Herausgeber: John N. Abelson, Melvin I. Simon
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• Themenwelt: Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
• Themenwelt: Technik
• ISBN-10: 0-08-049665-2 / 0080496652
• ISBN-13: 978-0-08-049665-8 / 9780080496658