Viral Gene Techniques (eBook)

eBook Download: PDF

1995 | 1. Auflage
416 Seiten
Elsevier Science (Verlag)
978-0-08-053640-8 (ISBN)

Infos

Viral Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The volume is concerned with methods for the analysis of viral genes and chromosomes: DNA viruses and RNA viruses including HIV are discussed.* Methods presented for ease of use and reasdy adaptation to new systems* Detailed experimental protocols included for:* Viral vectors - construction and use of DNA virus vectors (adenovirus, adeno-associated virus, vaccinia virus, Epstein - Barr virus)* DNA viruses - virus/host interactions, viral chromosomes , transcription regulation (viruses discussed include herpes simplex, hepatitis B, SV40, JC, Epstein-Barr, adenovirus)* Human Immunodeficiency Virus / retroviruses -quantitation of HIV-1 virus stock and RNA, retrovirus reverse transcription / integration, retrovirus-mediated cell fusion, use as cell lineage markers* RNA viruses - RNA virus assembly, analysis of RNA genomes, assays for RNA-binding proteins (viruses discussed include poliovirus, influenza virus, hepatitis delta virus)

Inhalt

Cover 1
Table of Contents 6
Contributors to Volume 7 10
Preface 16
Volumes in Series 18
Section I: Viral Vectors 20
Chapter 1. Adeno-Associated Virus: Integrating Vectors for Human Gene Therapy 22
Chapter 2. Techniques for Human Adenovirus Vector Construction and Characterization 32
Chapter 3. Construction and Isolation of Recombinant Adenoviruses with Gene Replacements 50
Chapter 4. Construction and Use of Recombinant Vaccinia Virus Vectors 64
Chapter 5. Epstein–Barr Viral Plasmid Vectors and Their Amplifiable Derivatives 84
Section II: DNA Viruses 106
Chapter 6. Methods to Study Epstein–Barr Virus and p53 Status in Human Cells 108
Chapter 7. The SV40 Minichromosome 120
Chapter 8. Site-Specific Integration of Multigenic Shuttle Plasmids into the Herpes Simplex Virus Type 1 Genome Using a Cell-Free Cre-lox Recombination System 133
Chapter 9. Strategies for Studying Transcription Regulation of Neurotropic Viruses in the Central Nervous System: Lesson from JC Virus 150
Chapter 10. Analysis of the Nuclear DNA-Binding Activity in Cells Overexpressing Hepatitis B Viral X and 3'-Truncated S Transactivators 171
Chapter 11. Investigations on Virus-Host Interactions: An Abortive System 186
Section III: Human Immunodeficiency Virus/Retroviruses 212
Chapter 12. Quantitation of Virus Stocks Produced from Cloned Human Immunodeficiency Virus DNA 214
Chapter 13. Quantitation of HIV-1 RNA in Plasma or Serum Samples 228
Chapter 14. Detection of Cell Fusion Mediated by the Envelopes of Human Retroviruses by Transactivation of a Reporter Gene 237
Chapter 15. Assembly of Recombinant Retroviral Gag Precursors into Pseudovirions in the Baculovirus–Insect Cell Expression System 256
Chapter 16. Retrovirus Reverse Transcription and Integration 273
Chapter 17. In Vitro Binding and Transcription Assays Using the Human T-Cell Leukemia Virus Type I Tax Protein 286
Chapter 18. Polymerase Chain Reaction-Based Techniques for Utilizing Retroviruses as Cell Lineage Markers 299
Section IV: RNA Viruses 316
Chapter 19. New Methods to Study Poliovirus Assembly and Encapsidation of Genomic RNA 318
Chapter 20. Molecular Studies of Hepatitis Delta Virus RNAs 334
Chapter 21. Systems to Express Recombinant RNA Molecules by the Influenza A Virus Polymerase in Vivo 348
Chapter 22. Analysis of Membrane Association in Vivo and Targeting of Viral Proteins in Polarized Epithelial Cells 362
Chapter 23. Cloning of Viral Double-Stranded RNA Genomes by Single Primer Amplification 378
Chapter 24. Using the RNA-Capture Assay to Assess the RNA-Binding Activity of Viral Proteins 392
Chapter 25. Protein RNA-Binding Activity Measured by Northwestern Blot Analysis: The Interferon-Inducible RNA-Dependent Protein Kinase PKR 407
Erratum 423
Index 424

Produkt-Details

• Erscheint lt. Verlag: 14.11.1995
• Mitarbeit: Herausgeber (Serie): Kenneth W. Adolph
• Sprache: englisch
• Themenwelt: Sachbuch/Ratgeber
• Themenwelt: Medizin / Pharmazie ► Allgemeines / Lexika
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
• Themenwelt: Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• Themenwelt: Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
• Themenwelt: Technik
• ISBN-10: 0-08-053640-9 / 0080536409
• ISBN-13: 978-0-08-053640-8 / 9780080536408