Enzymology Primer for Recombinant DNA Technology (eBook)

Hyone-Myong Eun (Autor)

eBook Download: PDF

1996 | 1. Auflage
702 Seiten
Elsevier Science (Verlag)
978-0-08-053113-7 (ISBN)

Infos

Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning.

Key Features
* Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology
* Presents information in an easily accessible format to serve as a quick reference source
* Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques

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Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. - Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology- Presents information in an easily accessible format to serve as a quick reference source- Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques

Inhalt

Front Cover 1
Enzymology Primer for Recombinant DNA Technology 4
Copyright Page 5
Contents 8
Preface 12
Acknowledgments 16
List of Abbreviations and Symbols 18
List of Journal Abbreviations 24
Chapter 1. Enzymes and Nucleic Acids: General Principles 26
I. Structure and Function of Enzymes 26
II. Structure and Function of Nucleic Acids 76
Chapter 2. Ligases 134
I. DNA Ligases 134
II. RNA Ligase: T4 RNA Ligase 158
Chapter 3. Nucleases 170
I. DNA Endonuclease: Deoxyribonuclease I 171
II. DNA/RNA Endonuclease: Staphylococcal Nuclease 185
III. RNA Endonucleases 194
IV. Single-Strand-Specific Endonucleases 229
V. DNA Exonucleases 240
Chapter 4. Restriction Endonucleases and Modification Methylases 258
I. General Description 258
II. Restriction Endonucleases 263
III. Methyltransferases (Methylases) 309
Chapter 5. Phosphatases and Polynucleotide Kinase 332
I. Phosphatases (Alkaline) 332
II. Polynucleotide Kinase: T4 Polynucleotide Kinase 358
Chapter 6. DNA Polymerases 370
I. General Description 370
II. DNA-Directed DNA Polymerases 376
III. RNA-Directed DNA Polymerases (Reverse Transcriptases) 452
IV. Template-Independent DNA Polymerase: Terminal Deoxynucleotidyltransferase 502
Chapter 7. RNA Polymerases 516
I. DNA-Directed RNA Polymerases 516
II. DNA-Independent RNA Polymerase: Poly(A) Polymerase 580
Chapter 8. Marker/Reporter Enzymes 592
A. ?-Galactosidase 593
B. ?-Lactamase 620
C. Chloramphenicol Acetyltransferase 638
D. Luciferases 652
Appendix A: Important Molecular Biological Methods 672
I. DNA Labeling 672
II. Nucleotide Sequencing 676
III. Polymerase Chain Reaction 682
IV. Site-Specific in Vitro Mutagenesis 684
Appendix B: Genotypes of Escherichia coli Strains 698
I. Genotypes of Some Frequently Used E. coli Strains 699
II. Useful Genetic Markers and Phenotypic Traits 702
Appendix C: Practical Guide for Enzyme Handling 708
Index 712

Produkt-Details

• Erscheint lt. Verlag: 3.6.1996
• Sprache: englisch
• Themenwelt: Sachbuch/Ratgeber
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• Themenwelt: Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
• Themenwelt: Technik ► Umwelttechnik / Biotechnologie
• ISBN-10: 0-08-053113-X / 008053113X
• ISBN-13: 978-0-08-053113-7 / 9780080531137