Regulators of G Protein Signaling, Part A (eBook)

David Siderovski (Herausgeber)

eBook Download: PDF

2004 | 1. Auflage
480 Seiten
Elsevier Science (Verlag)
978-0-08-049726-6 (ISBN)

Infos

This volume is an in-depth treatment of G-Protein Signaling, Part A, and will cover general methods of analysis of RGS protein analysis, including Expression and post-translational modification, Assays of GAP activity and allosteric control, Electrophysiological methods and RGS-insensitive Ga subunits, Mouse models of RGS protein action, Methods of RGS protein inhibition, and G-protein regulators of model organisms.

Table of Contents
-Expression and post-translational modification
-Assays of GAP activity and allosteric control
-Electrophysiological methods and RGS-insenstitive Ga subunits
-Mouse Models of RGS protein action
-Methods of RGS protein inhibition
-G-protein regulators of model organisms

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Regulators of G Protein Signaling, Part A is an in-depth treatment of G-Protein Signaling, and will cover general methods of analysis of RGS protein analysis, including Expression and post-translational modification, Assays of GAP activity and allosteric control, Electrophysiological methods and RGS-insensitive Ga subunits, Mouse models of RGS protein action, Methods of RGS protein inhibition, and G-protein regulators of model organisms. - Table of Contents- Expression and post-translational modification- Assays of GAP activity and allosteric control- Electrophysiological methods and RGS-insenstitive Ga subunits- Mouse Models of RGS protein action- Methods of RGS protein inhibition- G-protein regulators of model organisms

Inhalt

Cover 1
Table of Contents 6
Contributors to Volume 389 10
Preface 14
Volumes in Series 18
Section I: General Methods of RGS Protein Analysis Subsection A. Expression and Post-translational Modification 42
Chapter 1. Quantitative Real-Time Polymerase Chain Reaction Measurement of Regulators of G-Protein Signaling mRNA Levels in Mouse Tissues 44
Chapter 2. Regulation of Chemokine-Induced Lymphocyte Migration by RGS Proteins 56
Chapter 3. Role of Palmitoylation in RGS Protein Function 74
Subsection B: Assays of RGS Box Activity and Allosteric Control 97
Chapter 4. Fluorescence-Based Assays for RGS Box Function 97
Chapter 5. Application of RGS Box Proteins to Evaluate G-Protein Selectivity in Receptor-Promoted Signaling 112
Chapter 6. Allosteric Regulation of GAP Activity by Phospholipids in Regulators of G-Protein Signaling 130
Chapter 7. Assays of RGS Protein Modulation by Phosphatidylinositides and Calmodulin 146
Subsection C. Electrophysiological Methods and RGS-Insensitive Galpha Subunits 160
Chapter 8. Analyses of RGS Protein Control of Agonist- Evoked Ca2+ Signaling 160
Chapter 9. Measuring the Modulatory Effects of RGS Proteins on GIRK Channels 172
Chapter 10. Assays for G-Protein-Coupled Receptor Signaling Using RGS-Insensitive Galpha Subunits 196
Chapter 11. Use of RGS-Insensitive Galpha Subunits to Study Endogenous RGS Protein Action on G-Protein Modulation of N-Type Calcium Channels in Sympathetic Neurons 211
Chapter 12. Endogenous RGS Proteins Regulate Presynaptic and Postsynaptic Function: Functional Expression of RGS-Insensitive Galpha Subunits in Central Nervous System Neurons 231
Subsection D. Mouse Models of RGS Protein Action 246
Chapter 13. In Situ Hybridization Analysis of RGS mRNA Regulation and Behavioral Phenotyping of RGS Mutant Mice 246
Chapter 14. RGS-Insensitive G-Protein Mutations to Study the Role of Endogenous RGS Proteins 270
Subsection E. Methods of RGS Protein Inhibition 285
Chapter 15. Ribozyme- and siRNA-Mediated Suppression of RGS-Containing RhoGEF Proteins 285
Chapter 16. Structure-Based Design, Synthesis, and Activity of Peptide Inhibitors of RGS4 GAP Activity 307
Chapter 17. Yeast-Based Screening for Inhibitors of RGS Proteins 318
Section II: G-Protein Regulators of Model Organisms 344
Chapter 18. Genetic Analysis of RGS Protein Function in Caenorhabditis elegans 346
Chapter 19. Purification and In Vitro Functional Analysis of the Arabidopsis thaliana Regulator of G-Protein Signaling-1 361
Chapter 20. AtRGS1 Function in Arabidopsis thaliana 379
Chapter 21. Identification and Functional Analysis of the Drosophila Gene loco 391
Chapter 22. Analysis of the Roles of Pins and Heterotrimeric G Proteins in Asymmetric Division of Drosophila Neuroblasts 405
Chapter 23. Mathematical Modeling of RGS and G-Protein Regulation in Yeast 424
Chapter 24. Identification of Yeast Pheromone Pathway Modulators by High-Throughput Agonist Response Profiling of a Yeast Gene Knockout Strain Collection 440
Author Index 452
Subject Index 468

Produkt-Details

• Erscheint lt. Verlag: 25.11.2004
• Sprache: englisch
• Themenwelt: Sachbuch/Ratgeber
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
• Themenwelt: Technik
• ISBN-10: 0-08-049726-8 / 0080497268
• ISBN-13: 978-0-08-049726-6 / 9780080497266