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T Cell Hybridomas

Sources of Specific Mediators in the Immune System: Workshop: Papers

H V Boehmer, W Haas, G Kahler, F Melchers, J Zeuthen (Herausgeber)

Buch | Hardcover

276 Seiten

1982
Springer-Verlag Berlin and Heidelberg GmbH & Co. K
978-3-540-11535-9 (ISBN)

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For more than ten years cell fusion techniques have been applied in studies on various lymphocyte functions. Ig expression was first studied in hybrids obtained by fusing myeloma cells with fibroblasts (1) or lymphomas (2), both of which do not produce Ig, and with Ig producing myelomas (3) or human blood lymphocytes (4). Kohler and Milstein (5) fused a myeloma with spleen cells from immunized mice. Up to 10% of the hybrids obtained secreted antibodies specific for the immunizing antigen. This suggested that plasma cells preferenti ally fused with the myeloma cells, a finding which was of enormous practical value. It was found that both Band T lymphocytes could be fused with the T cell tumor BW5147, which is however not permissive for Ig synthesis (6). A very large number of T cell hybridomas were generated by fusing BW5147 with cell populations containing in vivo or in vitro activated cells (7). The hybrids showed no specific T cell functions and binding assays for T cell receptors were not available. In particular, no hybrids were obtained which expreS1ed specific cytolytic activity that could be tested in short-term Cr release assays (8). However, the frustrations expressed about these failures, published in January, 1978 (9), were relieved by Taniguchi and Miller's publication a few months later of T cell hybridomas producing antigen-specific suppressor factors (10). Unfortunately, their hybrids rapidly lost factor production."

Verlagsort	Berlin
Sprache	englisch
Themenwelt	Sachbuch/Ratgeber ► Gesundheit / Leben / Psychologie ► Lebenshilfe / Lebensführung
	Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
	Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
	Studium ► 2. Studienabschnitt (Klinik) ► Rechtsmedizin
	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
ISBN-10	3-540-11535-8 / 3540115358
ISBN-13	978-3-540-11535-9 / 9783540115359
Zustand	Neuware