Macrophages and Dendritic Cells
Humana Press Inc. (Verlag)
978-1-58829-972-7 (ISBN)
Authoritative and cutting-edge, Macrophages and Dendritic Cells: Methods and Protocols provides a timely and useful guide for both seasoned investigators and neophytes pursuing this imperative field of study.
Generation of Retroviral Macrophage cDNA Expression Libraries and Functional Screening for Surface Receptors.- Generation of Murine Growth Factor-Dependent Long-Term Dendritic Cell Lines to Investigate Host-Parasite Interactions.- Macrophage Activation: Classical Vs. Alternative.- Analysis of Macrophage Phagocytosis: Quantitative Assays of Phagosome Formation and Maturation Using High-Throughput Fluorescence Microscopy.- Detection of Activated Rab7 GTPase with an Immobilized RILP Probe.- Probing In Vivo Origins of Mononuclear Phagocytes by Conditional Ablation and Reconstitution.- Multivalent Protein Probes for the Identification and Characterization of Cognate Cellular Ligands for Myeloid Cell Surface Receptors.- Exploiting Fc Chimaeric Proteins for the Identification of Ligands Specific for the Mannose Receptor.- The Expression of Exogenous Genes in Macrophages: Obstacles and Opportunities.- Genetic Modification of Murine Dendritic Cells by RNA Transfection.- Recording Phagosome Maturation Through the Real-Time, Spectrofluorometric Measurement of Hydrolytic Activities.- Derivation and Characterization of Murine Alternatively Activated (M2) Macrophages.- Isolation and Culture of Human Hematopoietic Progenitors for Studies of Dendritic Cell Biology.- Generation, Culture and Flow-Cytometric Characterization of Primary Mouse Macrophages.- Analyzing Classical and Alternative Macrophage Activation in Macrophage/Neutrophil-Specific IL-4 Receptor-Alpha-Deficient Mice.- Analysis of Matrix Metalloproteinase Secretion by Macrophages.- Identification of Pathogen Receptors on Dendritic Cells to Understand their Function and to Identify New Drug Targets.- Stable Lentiviral Vector-Mediated Gene Silencing in Human Monocytic Cell Lines.- Use of Fluorescent Probes to Detect Lipid Signaling Intermediates in Macrophages.- Large-Scale Phagosome Preparation.- Coordinate Regulation of Sphingosine Kinase and Actin Dynamics.
From the reviews:
“This book, part of the Methods in Molecular Biology series, provides detailed descriptions of techniques that … may be adapted to individual research projects. … a book on state-of-the-art approaches to investigate the roles of macrophages and dendritic cells in health and disease to better understand their function in the host response. … useful for graduate students, postdoctoral fellows, and experienced investigators. … the protocols are described in great detail in this book, which should be helpful to anyone considering establishing a new research procedure.” (Marion C. Cohen, Doody’s Review Service, December, 2009)
Erscheint lt. Verlag | 1.4.2009 |
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Reihe/Serie | Methods in Molecular Biology ; 531 |
Zusatzinfo | XII, 368 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 193 x 260 mm |
Themenwelt | Studium ► Querschnittsbereiche ► Infektiologie / Immunologie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
ISBN-10 | 1-58829-972-4 / 1588299724 |
ISBN-13 | 978-1-58829-972-7 / 9781588299727 |
Zustand | Neuware |
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