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Practical Skills in Biomolecular Sciences - Rob Reed, Jonathan Weyers, Allan Jones, David Holmes

Practical Skills in Biomolecular Sciences

Buch | Softcover
568 Seiten
2007 | 3rd edition
Pearson/Education (Verlag)
978-0-13-239115-3 (ISBN)
CHF 71,90 inkl. MwSt
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Suitable for various Biomolecular Science students.
Practical Skills in Biomolecular Science, is an indispensable book for undergraduate students in the life sciences. The book provides useful support at all stages of a degree course and underpins any practical course in biochemistry, biomedical science, genetics, immunology and microbiology. It is also a valuable resource for teachers of biology in colleges and secondary schools. Laboratory and field studies are essential components of undergraduate training in biomolecular science. Practical work must be fully understood and effectively presented, but many students under-perform because they lack basic laboratory skills. This book, now in its third edition, continues to provide students with easy-to-use guidance for laboratory and field studies, but in addition it now covers broader transferable skills. As a result the new edition provides guidance and support over the entire range of a typical undergraduate course in biochemistry and biomedical science.

List of boxes

Preface

Guided tour

For the student

Acknowledgements

List of abbreviations



Study and examination skills

1 The importance of transferable skills

2 Managing your time

3 Working with others

4 Taking notes from lectures and texts

5 Learning and revising

6 Curriculum options, assessments and exams

7 Preparing your curriculum vitae



Information technology and library resources

8 Finding and citing published information

9 Evaluating information

10 Using online resources

11 Bioinformatics - Internet resources

12 Using spreadsheets

13 Word processors, databases and other packages



Communicating information

14 Organising a poster display

15 Giving a spoken presentation

16 General aspects of scientific writing

17 Writing essays

18 Reporting practical and project work

19 Writing literature surveys and reviews



Fundamental laboratory techniques

20 Your approach to practical work

21 Health and safety

22 Working with liquids

23 Basic laboratory procedures

24 Principles of solution chemistry

25 pH and buffer solutions

26 Introduction to microscopy

27 Setting up and using a light microscope



The investigative approach

28 Making and recording measurements

29 SI units and their use

30 Scientific method and design of experiments

31 Project work



Handling cells and tissues

32 Sterile technique and microbial culture

33 Isolating, identifying and naming microbes

34 Working with animal and plant tissues and cells

35 Culture systems and growth measurement

36 Homogenisation and fractionation of cells and tissues



Analytical techniques

37 Calibration and its application to quantitative analysis

38 Immunological methods

39 Radioactive isotopes and their uses

40 Light measurement

41 Basic spectroscopy

42 Advanced spectroscopy and spectrometry

43 Centrifugation

44 Chromatography - separation methods

45 Chromatography - detection and analysis

46 Principles and practice of electrophoresis

47 Advanced electrophoretic techniques

48 Electroanalytical techniques



Assaying biomolecules and studying metabolism

49 Analysis of biomolecules: fundamental principles

50 Assaying amino acids, peptides and proteins

51 Assaying lipids

52 Assaying carbohydrates

53 Assaying nucleic acids and nucleotides

54 Protein purification

55 Enzyme studies

56 Membrane transport processes

57 Photosynthesis and respiration



Genetics

58 Mendelian genetics

59 Bacterial and phage genetics

60 Molecular genetics I - fundamental principles

61 Molecular genetics II - PCR and related applications

62 Molecular genetics III - genetic engineering techniques



Analysis and presentation of data

63 Using graphs

64 Presenting data in tables

65 Hints for solving numerical problems

66 Descriptive statistics

67 Choosing and using statistical tests



Answers to study exercises

Index

Erscheint lt. Verlag 5.7.2007
Reihe/Serie Practical Skills
Sprache englisch
Maße 275 x 221 mm
Gewicht 1390 g
Themenwelt Naturwissenschaften Biologie Genetik / Molekularbiologie
ISBN-10 0-13-239115-5 / 0132391155
ISBN-13 978-0-13-239115-3 / 9780132391153
Zustand Neuware
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