Introduction to Fluorescence
CRC Press (Verlag)
978-1-032-26171-3 (ISBN)
- Noch nicht erschienen (ca. März 2025)
- Versandkostenfrei
- Auch auf Rechnung
- Artikel merken
This Second Edition of Introduction to Fluorescence helps readers acquire a sound understanding of basic fluorescence theory and practice. It describes general principles in a straightforward way and uses examples from a variety of disciplines to demonstrate them. Since the 1st Edition, significant advances in the field have appeared. For examples phasors, both lifetime and spectral phasors, have become very popular and so a chapter dedicated to this topic has been added. Furthermore, significant advances have been made in fluorescence microscopy methods, including super-resolution and single molecule techniques.
In color throughout, the book takes readers through the history of important discoveries to the most current advances. It introduces the fundamentals of the fluorescence phenomenon and gives detailed examples of fluorescence applications in the molecular life sciences, including biochemistry, biophysics, clinical chemistry and diagnostics, pharmaceutical science, and cell and molecular biology. The author presents the basic theories underlying the applications and offers in-depth information on practical aspects.
Numerous references are given in each chapter along with a list of particularly important references at the end of each chapter. The text incorporates more than 340 figures that clearly illustrate the concepts and gives the chemical structures of the most widely used fluorescent molecules. In addition, the appendix provides a "Rogue’s Gallery" of the most common errors and pitfalls to avoid.
David M. Jameson is professor in the Department of Cell and Molecular Biology at the John A. Burns School of Medicine, University of Hawaii at Manoa, having previously served there as professor and chairman of the Department of Biochemistry and Biophysics. He earned a PhD in biochemistry from the University of Illinois at Urbana-Champaign, where his graduate thesis advisor was Professor Gregorio Weber. Prior to his move to the University of Hawaii, he was an assistant professor in the Department of Pharmacology at the University of Texas Southwestern Medical Center at Dallas and a postdoctoral fellow at the University of Illinois at Urbana-Champaign and at the CNRS, University of Paris-South in Orsay, France. Dr. Jameson is the co-organizer of the International Weber Symposia on Innovative Fluorescence Methodologies in Biochemistry and Medicine, which have been held every three years since 1986. He was the recipient of the 2004 Gregorio Weber Award for Excellence in Fluorescence Theory/Application.
1 Introduction
2 Absorption of Light
3 Instrumentation
4 Emission and Excitation Spectra
5 Polarization/Anisotropy
6 Time-Resolved Fluorescence
7 Phasors
8 Quantum Yields and Quenching
9 Förster Resonance Energy Transfer (FRET)
10 Fluorescence Microscopy
11 Fluorophores
12 Intrinsic Protein Fluorescence
13 Appendix - Rogue’s gallery of fluorescence artifacts and errors
| Erscheint lt. Verlag | 27.3.2025 |
|---|---|
| Zusatzinfo | 2 Tables, black and white; 170 Line drawings, color; 104 Line drawings, black and white; 71 Halftones, color; 5 Halftones, black and white; 241 Illustrations, color; 109 Illustrations, black and white |
| Verlagsort | London |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Gewicht | 453 g |
| Themenwelt | Naturwissenschaften ► Chemie ► Physikalische Chemie |
| Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik | |
| ISBN-10 | 1-032-26171-4 / 1032261714 |
| ISBN-13 | 978-1-032-26171-3 / 9781032261713 |
| Zustand | Neuware |
| Haben Sie eine Frage zum Produkt? |
aus dem Bereich
