The Mitotic Spindle

Methods and Protocols

Tomomi Kiyomitsu (Herausgeber)

Buch | Hardcover

288 Seiten

2025 | 2025 ed.
Springer-Verlag New York Inc.
978-1-0716-4223-8 (ISBN)

Infos

This detailed volume presents methods for investigating mitotic spindle assembly, chromosome segregation, and cytokinesis. Split into six parts, the book examines advanced microscopes, spatiotemporal manipulation of the spindle and target molecules, quantitative live imaging, screening, centrosome manipulation and purification, and spindle positioning in synthetic or complicated contexts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding know pitfalls.



 



Authoritative and up-to-date, The Mitotic Spindle: Methods and Protocols serves as an ideal guide for researchers seeking a better system-level understanding of the mitotic spindle.



 



Chapters 4, 12, 17, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Inhalt

Super-Resolution Imaging of Mitotic Spindle Microtubules Using STED Microscopy.- Chromosome Counting at Meiosis and Mitosis of Mouse Oocytes and Embryos Using Super-Resolution Live-Cell Imaging and CRISPR/dCas9-Mediated Live-FISH.- Cell Division Imaging of Tobacco BY-2 Cells in a 3D Volume by Two-Photon Spinning-Disk Confocal Microscopy.- Spatial Statistics of Three-Dimensional Growth Dynamics of Spindle Microtubules.- A Centrifuge Polarizing Microscope (CPM) that Enables the Visualization of Intracellular Structures Under High Centrifugal Forces.- Manipulation of Spindle Position Using Magnetic Tweezers in Sea Urchin Embryos.- Photochemical Control of Cell Division Using a Photoswitchable CENP-E Inhibitor.- Quantitatively Analyzing the Morphological Growth Dynamics of Bipolar and Non-Bipolar Spindles Using Xenopus Egg Cytoplasmic Extract.- Visualization and Analyses of Cytoplasmic Streaming in C. elegans Zygotes.- 4D Microscopy and Tracking of Chromosomes and the Spindle in C. elegans Early Embryos.- Assessment of Chromosome Oscillations in Mammalian Cells by Live Cell Imaging.- Genetic Engineering and Screening Using Base Editing and Inducible Gene Knockout.- Systematic Identification of Microtubule Post-Translational Modification “Readers” by Quantitative Proteomics.- Comparative Pharmacological Analysis of Mitotic Inhibitors Using Isogenic Ploidy Series of HAP1 Cells.- A Method for Analyzing Acentrosomal Mitotic Spindles in Human Cells.- Isolation of Mitotic Centrosomes from Cultured Human Cells.- Synthetic Engineering of Cortical Polarity During Mitosis Using Designed Proteins.- High-Resolution Imaging of Spindle Orientation Dynamics in 3D Intestinal Organoids.- Time-Lapse Imaging of Asymmetric Spindle Positioning During Endothelial Tip Cell Division in Angiogenesis In Vivo.

Produkt-Details

• Erscheint lt. Verlag: 31.1.2025
• Reihe/Serie: Methods in Molecular Biology
• Zusatzinfo: 76 Illustrations, color; 6 Illustrations, black and white; Approx. 275 p. 81 illus., 76 illus. in color. With online files/update.
• Verlagsort: New York, NY
• Sprache: englisch
• Maße: 178 x 254 mm
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• ISBN-10: 1-0716-4223-5 / 1071642235
• ISBN-13: 978-1-0716-4223-8 / 9781071642238
• Zustand: Neuware