Schmidtea Mediterranea

Methods and Protocols

Buch | Softcover

280 Seiten

2024 | 2023 ed.
Springer-Verlag New York Inc.
978-1-0716-3277-2 (ISBN)

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This detailed volume examines fine-tuned methodologies using the planarian species, Schmidtea mediterranea. The book features experimental protocols covering topics from in situ hybridization, immunohistochemistry, cell dissociation and flow cytometry, to pipelines for the analysis of large datasets, as in genomics and transcriptomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Schmidtea mediterranea: Methods and Protocols provides both experts in the field and newcomers with the best possible toolbox for their everyday lab work utilizing this valuable model.

Building Phylogenies from Transcriptomic Data.- Isolation and Library Preparation of Planarian piRNAs.- Genome-Wide Analysis of Planarian piRNAs.- Combining Fluorescent In Situ Hybridization with Immunofluorescence and Lectin Staining in Planarians.- Colorimetric Whole-Mount In Situ Hybridization in Planarians.- Single Molecule Fluorescent In Situ Hybridization (smFISH) on Whole-Mount Planarians.- Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea.- Preparing Planarian Cells for High-Content Fluorescence Microscopy Using RNA In Situ Hybridization and Immunocytochemistry.- An RNA/DNA-Based Flow Cytometry Approach for Isolating Slow-Cycling Stem Cells.- ACME Dissociation-Fixation, Flow Cytometry, and Cell Sorting of Freshwater Planarian Cells.- Papain-Based Dissociation of Schmidtea mediterranea Cells.- Live Immunostaining and Flow Cytometry of Schmidtea mediterranea Cells.- Live Imaging in Planarians: Immobilization and Real-Time Visualization of Reactive Oxygen Species.- A Planarian Model System to Study Host-Pathogen Interactions.- TUNEL Staining in Sections of Paraffin-Enabled Planarians.- Quantitative Analysis of Planarian Pigmentation.- mRNA Transfection of S. mediterranea Cells for Luminescence Analysis.

Erscheinungsdatum	13.07.2024
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	50 Illustrations, color; 4 Illustrations, black and white; XI, 280 p. 54 illus., 50 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	Genomics and transcriptomics • immunohistochemistry • In situ Hybridization • Large dataset analysis • Planarians • Stem Cell Biology
ISBN-10	1-0716-3277-9 / 1071632779
ISBN-13	978-1-0716-3277-2 / 9781071632772
Zustand	Neuware