Cell Culture Techniques

Protocols, Methods, Tools and Processes

Shailendra Singh Shera (Autor)

Buch | Softcover

350 Seiten

2025
Academic Press Inc (Verlag)
978-0-323-85121-3 (ISBN)

Infos

Cell Culture Techniques: Protocols, Methods, Tools and Processes is the ideal laboratory reference to execute animal cell culture assays. Based on the author’s experience and practice, the selected methods represent the current status of the field. Cell culture practices covered in this book include the ones used both in academic and corporate lab setting. The initial part of the book guides the reader through the key decisions and challenges involved with setting up a cell culture laboratory. Serum-free media is then discussed as the way to provide cells the requirements for their growth. The next group of chapters cover the general procedures for cell culture, including selection of media, preparation and sterilization of equipment, tissue extraction or dissociation and initiation of the cell culture. The author then moves on to cover cell counting methods, cell proliferation assays, and scaling up animal cell culture. The chapters that follow discuss authentication, contamination detection by microbes and viruses. The next chapters cover stem cells, organoids, and spheroid cells. The last part of the book provides methods for cell separation, high-speed cell sorting, detection of cell cycle stages and then finally discusses biosafety aspects of cell culture. Providing readers a collection of over 50 animal cell culture laboratory methods, Cell Culture Techniques: Protocols, Methods, Tools and Processes is the ideal companion to bioscientists involved in animal research in academic settings and also to corporate based researchers looking for protocols, methods, tools and processes to improve their practice.

Autor

Shailendra Singh Shera is an Assistant professor in the Department of Biotechnology, Faculty of Engineering and Technology, RAMA University, Kanpur, Uttar Pradesh. He has received Bachelor in Technology in Biotechnology from Bundelkhand University, Master in Technology in Biotechnology from National Institute of Technology, Durgapur, West Bengal and has recently submitted his Ph.D in Biochemical Engineering at IIT (BHU), Varanasi. He is an early career researcher and has one-year teaching experience in cell culture science and technology. He has qualified various prestigious national level examinations such as National Eligibility Test (NET), Graduate Aptitude Test in Engineering (GATE), Biotech Industrial Training Programme (BITP) conducted by Biotech Consortium of India Limited (BCIL), Department of Biotechnology, Government of India. Mr. Shera has published more than 7 research articles in peer reviewed international science citation indexed (SCI) journals and 3 book chapters published by Elsevier and Springer publications and has presented his research at various prestigious national and international conferences.

Inhalt

1. Setting up a Cell Culture Laboratory
2. Development of Serum-Free Media
3. General Procedures for Cell Culture
4. Cell counting & proliferation assay
5. Scale up of animal cell culture
6. Fermenters and process control
7. Cell Line Authentication
8. Detection of Microbial and Viral Contaminants in Cell Lines
9. Stem cell culture
10. Culture of organoid cells
11. Culture of spheroid cells
12. Separation of Cell Populations Synchronized in Cell Cycle Phase
13. High-Speed Cell Sorting
14. Detection of Cell Cycle Stages in situ in Growing Cell Populations
15. Biosafety for cell biologists

Produkt-Details

• Erscheint lt. Verlag: 1.1.2025
• Verlagsort: Oxford
• Sprache: englisch
• Maße: 191 x 235 mm
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• ISBN-10: 0-323-85121-5 / 0323851215
• ISBN-13: 978-0-323-85121-3 / 9780323851213
• Zustand: Neuware