Plant Protein Secretion

Methods and Protocols

Liwen Jiang, Jinbo Shen, Caiji Gao, Xiangfeng Wang (Herausgeber)

Buch | Hardcover

250 Seiten

2024 | Second Edition 2024
Springer-Verlag New York Inc.
978-1-0716-4058-6 (ISBN)

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This volume explores the latest developments in protein secretion research in plants, as compared to yeast and mammalian systems. The chapters in this book present a diverse and thorough perspective of the field and cover topics such as bioinformatic analysis, proteomic studies, ultrastructural analysis, and genetic screening methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Plant Protein Secretion: Methods and Protocols, Second Edition is a valuable resource for researchers and students in the field of plant biology, and will inspire further advancements in our understanding of protein secretion in plant cells and beyond.

Conventional and Unconventional Protein Secretion in Yeast and Animal Cells.- An Overview of Protein Secretion in Plant Cells.- Bioinformatic Analysis for Identifying Transcription Factors Involved in Protein Secretion and Vacuole Formation.- Using AlphaFold2 and Molecular Dynamics Simulation to Model Protein Recognition.- Proteomic Analysis of Secreted Vesicle Proteins from Plant Cells.- Extracellular Vesicle Isolation and Mass-Spectrometry-Based Proteomic Analysis in Arabidopsis thaliana.- Polysaccharides Analysis of Cell Wall Using Carbohydrate Gel Electrophoresis.- Acetyl Bromide Method for Total Lignin Content Determination in Plant Biomass.- Utilizing Plant Protein Secretion System for Pharmaceutical Protein Production.- In Vitro Reconstitution of Plant COPII Vesicles.- Identification of Neighboring Proteins of Endosomal Regulators by Using TurboID-Based Proximity Labeling.- Quantification of Protein Dynamics in Growing Pollen Tubes via Whole Cell Kymograph Analysis.- Polar Localization Analysis of Anionic Phospholipids and Their Binding Proteins in Arabidopsis Pollen Tubes.- Vacuum-Based Agroinfiltration for Transient Gene Expression in Arabidopsis Seedlings.- Pharmaceutical Analysis in the Plant Endomembrane System.- Observation of Vacuoles in Arabidopsis Developing Pollen Grain with Whole-Cell Electron Tomography.- Whole-Cell Electron Tomography Analysis of Vacuole Biogenesis.- Microscopy Analysis of Autophagosomal Structures in Plant Cells.- Electron Microscopy Analysis of Membraneless Condensates in Plant Cells.- High-Pressure Freezing and Low-Temperature Processing of Seeds for Electron Microscopy and Electron Tomography.- Determination of the Cryo-EM Structure of ATG9 from Arabidopsis thaliana.- Genetic Screening of Factors in the Plant Protein Secretion.- Studying Autophagy and Senescence of Arabidopsis Stigmatic Cells.

Erscheinungsdatum	09.08.2024
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	49 Illustrations, color; 7 Illustrations, black and white; XII, 250 p. 56 illus., 49 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Botanik
Schlagworte	Bioinformatics • Electron Tomograms • homology modeling • Plant Golgi Stacks • Vacuole Formation
ISBN-10	1-0716-4058-5 / 1071640585
ISBN-13	978-1-0716-4058-6 / 9781071640586
Zustand	Neuware