Antifungal Immunity

Methods and Protocols

Buch | Softcover

226 Seiten

2024 | 2023 ed.
Springer-Verlag New York Inc.
978-1-0716-3201-7 (ISBN)

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This volume aims to help new investigators to the field by providing them with the protocols needed to set up clinically relevant animal models of fungal infection, as well as methods for in vivo immunology.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Antifungal Immunity: Methods and Protocols aims to be comprehensive guide for researchers in the field

Quantifying the mechanical properties of yeast Candida albicans using atomic force microscopy-based force spectroscopy.- Standardization of Galleria mellonella as an infection model for Malassezia furfur and Malassezia pachydermatis.- Mouse organotypic brain slice cultures: a novel model for studying neuroimmune responses to cryptococcal brain infections.- Zebrafish larvae as an experimental model of cryptococcal meningitis.- Immunological Analysis of Cryptococcal Meningoencephalitis in a Murine Model.- Mouse Model of Latent Cryptococcal Infection and Reactivation.- Adoptive transfer of Cryptococcus neoformans-specific CD4 T-cells to study anti-fungal lymphocyte responses in vivo.- Meningeal whole mounts for imaging CNS fungal infection.- An antibiotic-free model of Candida albicans colonisation of the murine gastrointestinal tract.- An experimental model of chromoblastomycosis caused by Fonsecaea sp species.- Modelling Chronic Coccidioidomycosis in Mice.- Mouse models of phaeohyphomycosis.- Genetic mouse models of Pneumocystis pneumonia.- Mouse models of Mucormycosis.- Bioluminescence imaging, a powerful tool to assess fungal burden in live mouse models of infection.- Micro-Computed Tomography to visualize and quantify fungal infection burden and inflammation in the mouse lung over time.

Erscheinungsdatum	08.05.2024
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	45 Illustrations, color; 3 Illustrations, black and white; XI, 226 p. 48 illus., 45 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Mykologie
Schlagworte	Animal Models • Fungal diseases • inflammation • Invasive fungal infections • In vivo immunology • Mycology
ISBN-10	1-0716-3201-9 / 1071632019
ISBN-13	978-1-0716-3201-7 / 9781071632017
Zustand	Neuware