Genome Editing in Animals
Springer-Verlag New York Inc.
978-1-0716-3018-1 (ISBN)
Authoritative and cutting-edge, Genome Editing in Animals: Methods and Protocols, Second Edition aims to be a useful practical guide to researches to help further their study in this field.
Construction and Evaluation of Zinc Finger Nucleases.- Updated overview of TALEN construction systems.- CRISPR/Cas9.- SNPD-CRISPR: Single Nucleotide Polymorphism-Distinguishable Repression or Enhancement of a Target Gene Expression by CRISPR System.- Utilizing large functional and population genomics resources for CRISPR/Cas perturbation experiment design.- Generation of genome-edited mice by cytoplasmic injection of CRISPR-Cas9 RNA.- Gene targeting in mouse embryonic stem cells via CRISPR/Cas9 ribonucleoprotein (RNP) mediated genome editing.- Generation of Knock-in Mouse by Genome Editing.- Introduction of genetic mutations into mice by Base Editor and Target-AID.- Genome editing in mouse and rat by electroporation.- Generation of floxed mice by sequential electroporation.- Efficient detection of flox mice using in vitro Cre recombination.- VCre/VloxP and SCre/SloxP as reliable site-specific recombination systems for genome engineering.- Efficient CRISPR/Cas9-Assisted Knockin ofLarge DNA Donors by Pronuclear Microinjection During S-phase in Mouse Zygotes.- Genome editing of murine liver hepatocytes by AAV vector-mediated expression of Cas9 in vivo.- Non-Viral Ex Vivo Genome-Editing in Mouse Bona Fide Hematopoietic Stem Cells with CRISPR/Cas9.- Genome editing of rat.- A simple and efficient method for generating KO rats using in vitro fertilized oocytes.- Editing the Genome of the Golden Hamster (Mesocricetus auratus).- Gene targeting in rabbits: single-step generation of knockout rabbits by microinjection of CRISPR/Cas9 plasmids.- Genome Editing of Pig.- GEEP method: an optimized electroporation-mediated gene-editing approach for establishment of knockout pig lines.- Genome editing mediated by primordial germ cell in chicken.- CRISPR–Cas9-mediated genome modifications in zebrafish.- Genome Editing of Medaka. -CRISPR-Cas9 based functional analysis in amphibians; Xenopus laevis, Xenopus tropicalis, and Pleurodeles waltl.- Genome Editing of Silkworms.- Improved genome editing in the ascidian Ciona with CRISPR/Cas9 and TALEN.- Genome editing of C. elegans.
| Erscheinungsdatum | 14.02.2024 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology |
| Zusatzinfo | 80 Illustrations, color; 25 Illustrations, black and white; XI, 399 p. 105 illus., 80 illus. in color. |
| Verlagsort | New York, NY |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie |
| Schlagworte | CRISPR/Cas9 • Embryonic Stem Cells • Knockout mice • Talen • zinc finger nucleases |
| ISBN-10 | 1-0716-3018-0 / 1071630180 |
| ISBN-13 | 978-1-0716-3018-1 / 9781071630181 |
| Zustand | Neuware |
| Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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