Archaea
Springer-Verlag New York Inc.
978-1-0716-2447-0 (ISBN)
Authoritative and cutting-edge, Archaea: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
Archaea: a goldmine for molecular biologists and evolutionists.- Progress and challenges in archaeal genetic manipulation.- Genetic manipulation of Haloferax species.- CRISPR interference as a tool to Repress Gene Expression in Haloferax volcanii.- Transformation Techniques for the Anaerobic Hyperthermophile Thermococcus kodakarensis.- Genetic Methods and Construction of Chromosomal Mutations in Methanogenic Archaea.- An interdomain conjugation protocol for plasmid-DNA transfer into Methanothermobacter thermautotrophicus ΔH.- Methods for Markerless Gene Deletion and Plasmid-Based Expression in Sulfolobus acidocaldarius.- A Rapid Targeted Gene Inactivation Approach in Sulfolobus islandicus.- Transposon Insertion Mutagenesis in hyperthermophilic crenarchaeon Sulfolobus islandicus.- Reprogramming CRISPR-mediated RNA interference for silencing of essential genes in Sulfolobales.- Progress and challenges in archaeal molecular biology.- ChIP-seq Occupancy Mapping of the Archaeal Transcription Machinery.- Conversion of ribosome-protected mRNA to DNA and deep sequencing for ribosome profiling in Haloferax volcanii.- Small RNA-sequencing library preparation for the halophilic archaeon Haloferax volcanii.- Quantitative Mass Spectrometry by SILAC in Haloferax volcanii.- Proteome turnover analysis in Haloferax volcanii by a heavy isotope multilabelling approach.- Proteomic sample preparation and data analysis in line with the Archaeal Proteome.- In vivo protein crosslinking and co-immunoprecipitation in Haloferax volcanii.- Proteolytic activity assays in haloarchaea.- Carotenoids from Haloarchaea: Extraction, fractionation and characterization.- Metal nanoparticles biosynthesis using the halophilic archaeon Haloferax volcanii.- Simplified enzymatic synthesis of 2-keto-3-deoxy-D-gluconate from D-gluconate using the gluconate dehydratase from Thermoproteus tenax.- Progress and Challenges in Archaeal Cellular Biology Analysis.- Methods to Analyze Motility in Eury- And Crenarchaea.- Immersed Liquid Biofilm and Honeycomb Pattern Formations in Haloferax volcanii.- Cost-effective and Versatile Analysis of Archaeal Surface Adhesion Under Shaking and Standing Conditions.- Cell Adhesion and Biofilm Formation Analysis.- BrdU incorporation and labelling of nascent DNA to Investigate Archaeal Replication using super-resolution imaging.- Isolation, Purification, and Characterization of Membrane Vesicles from Haloarchaea.- Archaeal Viruses: Production of Virus Particles and Vesicle-Like Viruses and Purification Using Asymmetrical Flow Field-Flow Fractionation.- Progress and Challenges in Studying the Ecophysiology of Archaea.- Reconstruction of Archaeal Genomes Assembly from Short-Read Metagenomes.- Outreach: Accessible and Insightful Scientific Learning Experiences Using the Microorganism Haloferax volcanii.
Erscheinungsdatum | 22.09.2023 |
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Reihe/Serie | Methods in Molecular Biology |
Zusatzinfo | 88 Illustrations, color; 10 Illustrations, black and white; XIV, 549 p. 98 illus., 88 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Schlagworte | ChIP/ChIP-seq • CRISPR • FISH/Immunofluorescence • halophiles • sulfolobales |
ISBN-10 | 1-0716-2447-4 / 1071624474 |
ISBN-13 | 978-1-0716-2447-0 / 9781071624470 |
Zustand | Neuware |
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