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Basic Cell Culture Protocols

Cheryl D. Helgason (Autor)

Media-Kombination

384 Seiten

2004 | 3rd Revised edition
Humana Press Inc.
978-1-58829-545-3 (ISBN)

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In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).

Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line Cheryl D. Helgason Detection of Mycoplasma Contaminations Cord C. Uphoff and Hans G. Drexler Eradication of Mycoplasma Contaminations Cord C. Uphoff and Hans G. Drexler Authentication of Scientific Human Cell Lines: Easy-to-Use DNA Fingerprinting Wilhelm G. Dirks and Hans G. Drexler Cytogenetic Analysis of Cell Lines Roderick A. F. MacLeod and Hans G. Drexler Human and Mouse Hematopoietic Colony-Forming Cell Assays Cindy L. Miller and Becky Lai Isolation and Culture of Murine Macrophages John Q. Davies and Siamon Gordon Isolation and Culture of Human Macrophages John Q. Davies and Siamon Gordon Development of T-Lymphocytes in Mouse Fetal Thymus Organ Culture Tomoo Ueno, Cunlan Liu, Takeshi Nitta, and Yousuke Takahama In Vitro Generation of Lymphocytes From Embryonic Stem Cells Renee F. de Pooter, Sarah K. Cho, and Juan Carlos Zuniga-Pflucker Hematopoietic Development of Human Embryonic Stem Cells in Culture Xinghui Tian and Dan S. Kaufman Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aorta-Gonads-Mesonephros Region Robert A. J. Oostendorp, Kirsty Harvey, and Elaine A. Dzierzak Culture of Human and Mouse Mesenchymal Cells Emer Clarke Isolation, Purification, and Cultivation of Murine and Human Keratinocytes Frizell L. Vaughan and Ludmila I. Bernstam Isolation and Culture of Primary Human Hepatocytes Edward L. LeCluyse, Eliane Alexandre, Geraldine A. Hamilton, Catherine Viollon-Abadie, D. James Coon, Summer Jolley, and Lysiane Richert Primary Kidney Proximal Tubule Cells Mary Taub Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect Progenitor Activity John Stingl, Joanne T. Emerman, and Connie J. Eaves Generation and Differentiation of Neurospheres From Murine Embryonic Day 14 Central Nervous System Tissue Sharon A. Louis and Brent A. Reynolds Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells Gabi Shefer and Zipora Yablonka-Reuveni Adult Ventricular Cardiomyocytes: Isolation and Culture Klaus-Dieter Schluter and Daniela Schreiber Isolation and Culture of Primary Endothelial Cells Bruno Larrivee and Aly Karsan Studying Leukocyte Rolling and Adhesion In Vitro Under Flow Conditions Susan L. Cuvelier and Kamala D. Patel Isolation and Characterization of Side Population Cells Margaret A. Goodell, Shannon McKinney-Freeman, and Fernando D. Camargo Scalable Production of Embryonic Stem Cell-Derived Cells Stephen M. Dang and Peter W. Zandstra Index

Erscheint lt. Verlag	15.10.2004
Reihe/Serie	Methods in Molecular Biology ; v. 290
Zusatzinfo	biography
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Gewicht	1 g
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	1-58829-545-1 / 1588295451
ISBN-13	978-1-58829-545-3 / 9781588295453
Zustand	Neuware