Pseudomonas aeruginosa

Methods and Protocols

Giovanni Bertoni, Silvia Ferrara (Herausgeber)

Buch | Hardcover

246 Seiten

2023 | 2024 ed.
Springer-Verlag New York Inc.
978-1-0716-3472-1 (ISBN)

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This book aims to provide methods, protocols, and discussion topics for those who wish to examine in depth the molecular mechanisms of adaptation and versality of bacteria and would like to envisage their evolution responses in the fast changing Antropocene.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Pseudomonas aeruginosa: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

CRISPR/Cas9-based genome editing of Pseudomonas aeruginosa.- Investigating Pseudomonas aeruginosa Gene Function During Pathogenesis using Mobile-CRISPRi.- Engineering green-light-responsive heterologous gene expression in Pseudomonas.- Fluorescence-based evaluation of cyclic di-GMP levels in Pseudomonas aeruginosa.- Whole-cell biosensors for qualitative and quantitative analysis of quorum sensing signal molecules and the investigation of quorum quenching agents.- A Pseudomonas aeruginosa-suitable fluorescent reporter system for analyzing small RNA-mediated regulation of target mRNAs.- The Pseudomonas aeruginosa resistome: permanent and transient antibiotic resistance, an overview.- Biosensors for inducers of transient antibiotic resistance.- Pseudomonas aeruginosa soluble pyocins as antibacterial weapons.- Assays for studying Pseudomonas aeruginosa secreted proteases.- Single microcolony diffusion analysis in Pseudomonas aeruginosa biofilms.- Broad genome sequencing of environmental and clinical strains and genotyping.- Genome-scale analysis of the structure and function of RNA pathways and networks in Pseudomonas aeruginosa.- In-depth quantitative proteomics analysis of the Pseudomonas aeruginosa secretome. Improving the predictive value of preclinical mouse models of Pseudomonas aeruginosa respiratory infection to evaluate antibiotic efficacy.- Emerging in vitro models for the study of infection and pathogenesis of Pseudomonas aeruginosa and testing of antibacterial agents.

Erscheinungsdatum	13.10.2023
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	40 Illustrations, color; 11 Illustrations, black and white; X, 246 p. 51 illus., 40 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	Atomic force microscopy • Confocal laser scanning microscopy • CRISPR/Cas9 • pangenome • Tn-Seq
ISBN-10	1-0716-3472-0 / 1071634720
ISBN-13	978-1-0716-3472-1 / 9781071634721
Zustand	Neuware