Genetically Incorporated Non-Canonical Amino Acids (eBook)

Methods and Protocols

Simon J. Elsasser, Yu-Hsuan Tsai (Herausgeber)

eBook Download: EPUB

2023
Springer US (Verlag)
978-1-0716-3251-2 (ISBN)

Infos

This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.

Produkt-Details

• Erscheint lt. Verlag: 5.6.2023
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Chemie
• ISBN-10: 1-0716-3251-5 / 1071632515
• ISBN-13: 978-1-0716-3251-2 / 9781071632512