Phagocytosis and Phagosomes
Springer-Verlag New York Inc.
978-1-0716-3337-3 (ISBN)
Authoritative and up-to-date, Phagocytosis and Phagosomes: Methods and Protocols, Second Edition serves as a vital resource for both experts in the field as well as for investigators delving into phagocytosis and phagosome maturation for the first time.
Bacterial Binding, Phagocytosis, and Killing: Measurements Using Colony Forming Units.- Analysis of Human and Mouse Neutrophil Phagocytosis by Flow Cytometry.- Quantifying Phagocytosis by Immunofluorescence and Microscopy.- Methods for Quantitative Efferocytosis Assays.- Analysis of Efferocytic Receptor Dynamics and Synapse Formation in a Frustrated Efferocytosis Model.- Super-Resolution Spinning-Disk Confocal Microscopy Using Optical Photon Reassignment (SoRa) to Visualize the Actin Cytoskeleton in Macrophages.- Filamentous Bacteria as Targets to Study Phagocytosis.- The Derivation and Use of HoxB8-Driven Conditionally Immortalized Macrophages.- Quantitative Immunofluorescence to Study Phagosome Maturation and Resolution.- Approaches to Measuring Reductive and Oxidative Events in Phagosomes.- Measuring Phagosomal pH by Fluorescence Microscopy.- Multiplexed Phagosomal Assays for the Detection and Quantification of Bidirectional Exchange Between the Phagolysosomal Lumen and Extracellular Space.- Quantitative Spatio-Temporal Analysis of Phagosome Maturation in Live Cells.- Measurement of Salmonella enterica Internalization and Vacuole Lysis in Epithelial Cells.- Microscopy-Based Tracking and Quantification Methods to Study Phagosome Resolution.- Isolation of Polystyrene Bead-Induced Phagosomes for Western Blotting.- Biochemically Reconstituted Fusion of Phagosomes with Endosomes and Lysosomes.- An In Vitro System to Analyze Generation and Degradation of Phagosomal Phosphatidylinositol Phosphates.- Examining Phagosomal Pattern Recognition Receptor-Dependent Signaling and Antigen MHC-II Presentation from Phagosomes in Dendritic Cells.- Examining the Kinetics of Phagocytosis-Coupled Inflammasome Activation in Murine Bone Marrow-Derived Dendritic Cells.- Analysis of LC3-Associated Phagocytosis and Antigen Presentation in Macrophages and B Cells.- Visualizing Phagocytic Cargo In Vivo from Engulfment to Resolution in Caenorhabditis elegans.- Assessing the Phagosome Proteome by Quantitative Mass Spectrometry.- Using Ion Substitution and Fluid Indicators to Monitor Macropinosome Dynamics in Live Cells.
Erscheinungsdatum | 29.06.2023 |
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Reihe/Serie | Methods in Molecular Biology |
Zusatzinfo | 53 Illustrations, color; 22 Illustrations, black and white; XI, 387 p. 75 illus., 53 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
ISBN-10 | 1-0716-3337-6 / 1071633376 |
ISBN-13 | 978-1-0716-3337-3 / 9781071633373 |
Zustand | Neuware |
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