Flower Development

Methods and Protocols

José Luis Riechmann, Cristina Ferrándiz (Herausgeber)

Buch | Hardcover

588 Seiten

2023 | Second Edition 2023
Springer-Verlag New York Inc.
978-1-0716-3298-7 (ISBN)

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This second edition details new and updated protocols for experimental approaches that are currently used to study the formation of flowers. Chapters guide readers on genetic methods, phenotypic analyses, genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Flower Development: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Flower development in Arabidopsis.- Flower development in the Solanaceae.- The ABC of flower development in monocots: the model of rice spikelet.- Model species to investigate the origin of flowers.- Hormones and flower development in Arabidopsis.- Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors.- Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development.- Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and rice.- Isolation of meiocytes and cytological analyses of male meiotic chromosomes in soybean, lettuce and maize.- Genetic and phenotypic analyses of carpel development in Arabidopsis.- Genetic and Phenotypic Analysis of Ovule Development in Arabidopsis.- Floral induction systems for the study of Arabidopsis flower development.- Protoplasting and Fluorescence-activated cell sorting of the shoot apical meristem cell types.- Protoplast Isolation for Plant Single-Cell RNA-seq.- Plant Nuclei Isolation for Single-nucleus RNA Sequencing.- Isolation of nuclei tagged in specific cell types (INTACT) in Arabidopsis.- RNA in situ hybridization on plant tissue sections: expression analysis at cellular resolution.- The GUS reporter system in flower development studies.- Expression and functional studies of leaf, floral and fruit developmental genes in non-model species.- Gene expression analysis by quantitative real-time PCR for floral tissues.- Misexpression approaches for the manipulation of flower development.- Genomic approaches for the study of flower development in floriculture crops.- Multi-omics methods applied to flower development.- Peptidomics methods applied to the study of flower development.- Quantifying gene expression domains in plant shoot apical meristems.- A NanoLuc-based transactivation assay in plants.- A hands-on guide to generate spatial gene expression profiles by integrating scRNA-seq and 3D reconstructed microscope-based plant structures.

Erscheinungsdatum	09.08.2023
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	67 Illustrations, color; 19 Illustrations, black and white; XV, 588 p. 86 illus., 67 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Botanik
Schlagworte	Anther Morphogenesis • Arabidopsis • Carpel Development • Floral Meristem Development • INTACT • Trap
ISBN-10	1-0716-3298-1 / 1071632981
ISBN-13	978-1-0716-3298-7 / 9781071632987
Zustand	Neuware