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PCR Protocols in Molecular Toxicology - John P. Vanden Heuvel

PCR Protocols in Molecular Toxicology

Buch | Softcover
252 Seiten
1997
Crc Press Inc (Verlag)
978-0-8493-3344-6 (ISBN)
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Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:

Quantitative reverse transcriptase PCR and methods to examine gene expression

Differential display cloning

Cloning and library screening by PCR

Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes

Basic, non-PCR based molecular biology methods

PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.

Vanden Heuvel, John P.

Chapter 1. The Basics of the Polymerase Chain Reaction -- /William B. Mattes -- Chapter 2. Analysis of Gene Expression -- /John P. Vanden Heuoel -- Chapter 3. Differential Display PCR -- /J. Christopher Corton -- Chapter 4 . Cloning by PCR -- /John P. Vanden Heuuel -- Chapter 5. Genotype Analysis -- /Douglas A. Bell and Gary Pittman -- Chapter 6 . General Molecular Biology Techniques -- /John P. Vanden Heuvel -- Appendix I — List of Suppliers -- Appendix II — Companies, Online Catalogs, and Internet Resources -- Index.

Erscheint lt. Verlag 24.9.1997
Reihe/Serie Methods in Life Sciences - Toxicology Section
Zusatzinfo 70 Tables, black and white; 3 Halftones, black and white; 3 Illustrations, black and white
Verlagsort Bosa Roca
Sprache englisch
Maße 156 x 234 mm
Gewicht 376 g
Einbandart Paperback
Themenwelt Studium 2. Studienabschnitt (Klinik) Pharmakologie / Toxikologie
Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Genetik / Molekularbiologie
ISBN-10 0-8493-3344-X / 084933344X
ISBN-13 978-0-8493-3344-6 / 9780849333446
Zustand Neuware
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