Single-Domain Antibodies
Springer-Verlag New York Inc.
978-1-0716-2077-9 (ISBN)
Authoritative and cutting-edge, Single-Domain Antibodies: Methods and Protocols aims to be a useful, practical guide to help researchers further their studies in this field.
Nanobodies: From Serendipitous Discovery of Heavy Chain-Only Antibodies in Camelids to a Wide Range of Useful Applications.- Overview, Generation, and Significance of Variable New Antigen Receptors (VNARs) as a Platform for Drug and Diagnostic Development.- Llama DNA Immunization and Isolation of Functional Single-Domain Antibody Binders.- Preparation of Immune and Synthetic VNAR Libraries as Sources of High-Affinity Binders.- Isolation of Single-Domain Antibodies to Transmembrane Proteins Using Magnetized Yeast Cell Targets.- A Transgenic Heavy Chain IgG Mouse Platform as a Source of High Affinity Fully Human Single-Domain Antibodies for Therapeutic Applications.- Cytoplasmic Production of Nanobodies and Nanobody-Based Reagents by Co-Expression of Sulfhydryl Oxidase and DsbC Isomerase.- Small Scale Secretory VHH Expression in Saccharomyces cerevisiae.- Production of Single-Domain Antibodies in Pichia pastoris.- Production of Designer VHH-Based Antibodies in Plant.- Assessing the Aggregation Propensity of Single-Domain Antibodies Upon Heat-Denaturation Employing the ΔTm Shift.- Facile Affinity Maturation of Single-Domain Antibodies Using Next-Generation DNA Sequencing.- Engineering pH-Sensitive Single-Domain Antibodies.- Humanization of Camelid Single-Domain Antibodies.- Creation of Multimeric Single-Domain Antibodies Using Bacterial Superglues.- Introducing Cysteines into Nanobodies for Site-Specific Labelling.- Affinity-Guided Site Selective Labelling of Nanobodies With Aldehyde Handles.- Cytoplasmic Expression of Nanobodies with Formylglycine Generating Enzyme Tag and Conversion to a Bio-Orthogonal Aldehyde Group.- Site-Specific Fluorescent Labelling, Single-Step Immunocytochemistry, and Delivery of Nanobodies into Living Cells.- Design and Validation of Site-Specifically Labelled Single-Domain Antibody-Based Tracers forIn Vivo Fluorescence Imaging and Image-Guided Surgery..- Design and Preparation of Photobodies: Light-Activated Single-Domain Antibody Fragments.- Visualizing Filoviral Nucleoproteins Using Nanobodies Fused to the Ascorbate Peroxidase Derivatives APEX2 and dEAPX.- Development of Glypican-2 Targeting Single-Domain Antibody CAR T Cells for Neuroblastoma.- Single-Domain Antibodies for Intracellular Toxin Neutralization.- Generation of Single-Domain Antibody-Based Recombinant Immunotoxins.- X-Ray Crystal Structure Analysis of VHH-Protein Antigen Complexes.- Functionalization of Magnetic Beads with Biotinylated Nanobodies for MALDI-TOF/MS-Based Quantitation of Small Analytes.- Nanobody-Based Assays for the Detection of Environmental and Agricultural Contaminants.- Peptide Tag-Specific Nanobodies for Studying Proteins in Live Cells.- Nanobody-Based GFP Traps to Study Protein Localization and Function in Developmental Biology.- Optogenetic Activation of Intracellular Nanobodies.
| Erscheinungsdatum | 18.02.2023 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology |
| Zusatzinfo | 84 Illustrations, color; 11 Illustrations, black and white; XIV, 612 p. 95 illus., 84 illus. in color. |
| Verlagsort | New York, NY |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| ISBN-10 | 1-0716-2077-0 / 1071620770 |
| ISBN-13 | 978-1-0716-2077-9 / 9781071620779 |
| Zustand | Neuware |
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