Imaging and Quantifying Neuronal Autophagy

Buch | Softcover

150 Seiten

2022 | 1st ed. 2022
Springer-Verlag New York Inc.
978-1-0716-1591-1 (ISBN)

Artikel merken

The volume aims to explore the dynamic nature of the autophagy pathway, and the latest techniques that allow researchers to capture and quantify this process in neurons. The chapters in this volume cover topics such as fundamental, historical, and functional approaches that began in baker’s yeast; Saccharomyces cerevisiae; the role of both electron microscopy and live-cell imaging using fluorescently tagged autophagy proteins; and the rate of puncta appearance and its correlation with the rate of autophagosome formation. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and practical, Imaging and Quantifying Neuronal is a valuable resource that provides insights into the power of microscopy tools, live-cell imaging, and photoactivation and correlative techniques.

Quantification of Autophagosome Size and Formation Rate by Electron and Fluorescence Microscopy in Baker’s Yeast.- Ultrastructure of the Macroautophagy Pathway in Mammalian Cells.- Live Imaging of Autophagosome Biogenesis and Maturation in Primary Neurons.- Monitoring Autophagic Activity In Vitro and In Vivo using the GFP-LC3-RFP-LC3∆G Probe.- Measuring Autophagic Flux in Neurons by Optical Pulse Labeling.- Measuring Autophagosome Flux.- Measurement of Neuronal Tau Clearance In Vivo.- Methods for Studying Axonal Autophagosome Dynamics in Adult Dorsal Root Ganglion Neurons.- Imaging and Quantifying Neuronal Autophagy to Determine the Autophagy Contribution to Neuronal and Dendritic Morphogenesis.- Correlative Light and Electron Microscopy (CLEM): Bringing Together the Best of Both Worlds to Study Neuronal Autophagy.

Erscheinungsdatum	26.08.2022
Reihe/Serie	Neuromethods ; 171
Zusatzinfo	27 Illustrations, color; 6 Illustrations, black and white; XII, 150 p. 33 illus., 27 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Zoologie
Schlagworte	block-face electron • correlative microscopy • Fluorescence probes • Fusion Inhibitors • quantitative morphometric analyses
ISBN-10	1-0716-1591-2 / 1071615912
ISBN-13	978-1-0716-1591-1 / 9781071615911
Zustand	Neuware