Challenges in Biological Cryo Electron Microscopy

Faraday Discussion 240

Buch | Hardcover

338 Seiten

2022
Royal Society of Chemistry (Verlag)
978-1-83916-425-5 (ISBN)

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Developments in cryo-electron microscopy are creating new opportunities within structural biology and there is currently great interest in developing cryo-EM as a core tool for atomic level structural biology. Many structural techniques can give atomic or near atomic level information, but lack the ability to study proteins within a near-native environment, for example within a cellular compartment. Cryo-EM provides this opportunity, but despite the recent massive improvements in single particle cryo-EM, obtaining sub-2Å structural information is still a major challenge. Cryo-electron microscopy has undergone significant developments in microscope design, camera technology and data processing regimes, but there are significant challenges that remain and opportunities to explore, many of which must be tackled by the community as a whole, rather than by individual groups. For example, sample preparation is central to electron microscopy and is currently a significant bottleneck in many experiments, and there are significant problems with ensuring the integrity of the field in terms of dealing with inherently low signal-to-noise images.

This volume brings together leading researchers from the UK and the international cryo-electron microscopy community to discuss current developments and new challenges in the field.

In this volume the topics covered include:

Sample preparation in single particle cryo-EM
Pushing the limits in single particle cryo-EM
Tomographic analysis, CLEM
Map/model validation and machine learning in EM

Faraday Discussions documents a long-established series of Faraday Discussion meetings which provide a unique international forum for the exchange of views and newly acquired results in developing areas of physical chemistry, biophysical chemistry and chemical physics. The papers presented are published in the Faraday Discussion volume together with a record of the discussion contributions made at the meeting. Faraday Discussions therefore provide an important record of current international knowledge and views in the field concerned. The latest (2020) impact factor of Faraday Discussions is 4.008.

Sample preparation in single particle cryo-EM;Pushing the limits in single particle cryo-EM;Tomographic analysis, CLEM;Map/model validation and machine learning in EM

Erscheinungsdatum	13.12.2022
Reihe/Serie	Faraday Discussions ; Volume 240
Mitarbeit	Sonstige Mitarbeit: Royal Society of Chemistry
Verlagsort	Cambridge
Sprache	englisch
Maße	156 x 234 mm
Gewicht	730 g
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Themenwelt	Naturwissenschaften ► Chemie ► Analytische Chemie
ISBN-10	1-83916-425-5 / 1839164255
ISBN-13	978-1-83916-425-5 / 9781839164255
Zustand	Neuware