Confocal Microscopy

Methods and Protocols

Joseph Brzostowski, Haewon Sohn (Herausgeber)

Buch | Softcover

363 Seiten

2021 | 1st ed. 2021
Springer-Verlag New York Inc.
978-1-0716-1404-4 (ISBN)

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This volume provides a wide range of imaging protocols that can be tailored to specific organisms or cell-types. Chapters guide readers through fixed-cell, live-cell, phenotype screening, super-resolution, intravital imaging techniques, and fluorescence life-time imaging microscopy (FLIM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Confocal Microscopy: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Advances in Confocal Microscopy and Selected Applications.- Choosing Fluorescent Probes and Labeling Systems.- General Considerations for Acquiring a Three-color Image by Laser Scanning Confocal Microscopy.- Microfabricated devices for confocal microscopy on biological samples.- Zeiss Airyscan: Optimizing Usage for Fast, Gentle, Super-resolution Imaging.- High-resolution multicolor imaging of mitochondria in lymphocytes.- Protein-Retention Expansion Microscopy (ExM): Scalable and Convenient Super-Resolution Microscopy.- Analysis of B Cell Receptor-Mediated Antigen Extraction by B lymphocytes from Plasma Membrane Sheets Using Confocal Microscopy.- Analysis of Intracellular Vesicles in B Lymphocytes: Antigen Traffic in the Spotlight.- Visualizing Key Signaling Components of Macropinocytosis and Phagocytosis Using Confocal Microscopy in the Model Organism Dictyostelium discoideum.- Imaging GPCR-mediated Signal Events Leading to Chemotaxis and Phagocytosis.- High-throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains.- Visualizing the Dynamics of T Cell-Dendritic Cell Interactions in Intact Lymph Nodes By Multiphoton Confocal Microscopy.- Studying Neuronal Biology Using Spinning Disc Confocal Microscopy.- Method for Acute Intravital Imaging of the Large Intestine in Live Mice.- Fluorescence Lifetime Imaging as a Non-invasive Tool to Study Plasmodium Falciparum Metabolism.- Developing Analysis Protocols for Monitoring Intracellular Oxygenation using Fluorescence Lifetime Imaging of Myoglobin-mCherry.- FLIM Imaging for Metabolic Studies in Live Cells.- A Step by Step Guide to Instant Structured Illumination Microscopy (ISIM).

Erscheinungsdatum	30.05.2022
Reihe/Serie	Methods in Molecular Biology ; 2304
Zusatzinfo	73 Illustrations, color; 15 Illustrations, black and white; XI, 363 p. 88 illus., 73 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Allgemeines / Lexika
	Naturwissenschaften ► Biologie ► Zellbiologie
	Naturwissenschaften ► Chemie ► Analytische Chemie
	Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
Schlagworte	Biological Microscopy • Cellular imaging • fluorescent probes • histo-cytometry • microfluidics • RNA probes
ISBN-10	1-0716-1404-5 / 1071614045
ISBN-13	978-1-0716-1404-4 / 9781071614044
Zustand	Neuware