Translational Research Methods in Neurodevelopmental Disorders

Stéphane Martin, Frédéric Laumonnier (Herausgeber)

Buch | Hardcover

264 Seiten

2022 | 1st ed. 2022
Springer-Verlag New York Inc.
978-1-0716-2568-2 (ISBN)

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This volume explores the latest techniques used to study neurodevelopmental diseases (NDD) that range from molecular aspects to integrated research approaches and brain imaging in living rodents. Chapters in this book cover topics such as protocols to deliver shRNA in vitro and in vivo using lentiviral particles to knock-down specific protein expression; experimental procedures to use recombinant fluorescent probes to visualize endogenous proteins at the mammalian synapse; CRISPR/Cas9 toolkit to assess either gain- or loss-of-gene function in brain organoids; detailed protocols to use in vivo manipulations to correct the behavioral phenotypes in cognitive disorder mouse lines; and experimental approaches to genetically engineer macaque models of NDDs and investigate how genetic predisposition may cause neural and functional alterations. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results inyour laboratory.

Cutting-edge and practical, Translational Research Methods in Neurodevelopmental Disorders is a valuable resource for all researchers who want to learn more about this important and developing field.

Using Lentiviral shRNA Delivery to Knock-Down Proteins in Cultured Neurons and In Vivo.- Synaptosomal Preparation and Its Application in Preclinical Studies.- Assessing the Nanoscale Organization of Excitatory and Inhibitory Synapses using Recombinant Probes to Visualize Endogenous Synaptic Proteins.- Agonist-Induced Functional Analysis and Cell Sorting (aiFACS) to Select and Characterize Interneurons during Brain Development.- A CRISPR/Cas9-Based Toolkit to Test Gain- and Loss-of-Gene Function in Brain Organoids.- Assessment of Adult Mouse Brain NeuroAnatomical Phenotypes using Quantitative and Precision Histology.- Leveraging Microelectrode Array Technology for Phenotyping Stem Cell-Derived Neurodevelopmental Disease Models.- Humanized Chimeric Mouse Models to Study Human Neural Development and Pathogenesis of Brain Diseases.- In Vivo Manipulations to Correct for Behavioral Phenotypes in Cognitive Disorder Mouse Models.- Assessing Dyadic Social Interactions in Rodent Models of Neurodevelopmental Disorders.- Central and Peripheral Explorations by Metabolomics to Study Neurodevelopmental Disorders.- Genetic Engineering of Non-Human Primates Models for Studying Neurodevelopmental Disorders.

Erscheinungsdatum	24.10.2022
Reihe/Serie	Neuromethods ; 185
Zusatzinfo	44 Illustrations, color; 6 Illustrations, black and white; XIII, 264 p. 50 illus., 44 illus. in color. With online files/update.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Zoologie
Schlagworte	autism spectrum disorders • Electrophysiolopgical methods • intellectual disability • neurotransmitter release assays • Next generation sequencing
ISBN-10	1-0716-2568-3 / 1071625683
ISBN-13	978-1-0716-2568-2 / 9781071625682
Zustand	Neuware