Single Cell ‘Omics of Neuronal Cells

Jonathan V. Sweedler, James Eberwine, Scott E. Fraser (Herausgeber)

Buch | Hardcover

294 Seiten

2022 | 1st ed. 2022
Springer-Verlag New York Inc.
978-1-0716-2524-8 (ISBN)

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This volume discusses the latest techniques used in the diverse fields of single cell ‘omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory.

Cutting-edge and comprehensive, Single Cell ‘Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.

Singe Cell aRNA-Seq for Transcriptomics and Cellular Phenotyping.- Single Cell Genomic and Epigenomic DNA Analyses.- Single-Cell Proteome Profiling of Neuronal Cells.- Joint Analysis of Transcriptome and Proteome Measurements in Single Cells with TotalVI.- Single-Cell Mass Spectrometry of Metabolites and Proteins for Systems and Functional Biology.- High-Throughout Image-Guided Microprobe Mass Spectrometric Analysis of Single Cells.- Single-Cell Resolution Immunoblotting.- Quantifying Enzyme Activity and Gene Expression within Single-Cells using a Multiplexed Capillary Electrophoresis Platform.- Quantitative Chemical Imaging at the Cell Level-SIMS, Fluorescence, and Correlative Techniques.- Electrophysiology and Single Cells.- Genetically Encoded Biosensors Reveal Spatiotemporal Dynamics and Cellular Heterogeneity of Neuronal Cells.

Erscheinungsdatum	17.08.2022
Reihe/Serie	Neuromethods ; 184
Zusatzinfo	56 Illustrations, color; 2 Illustrations, black and white; XIV, 294 p. 58 illus., 56 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Zoologie
Schlagworte	high-throughput single cell isolation • Mass Spectrometry • nano-LC-MS/MS • NextGen Sequencing • Proteomics • RNA amplification • single cell transcriptome • Transcriptomics
ISBN-10	1-0716-2524-1 / 1071625241
ISBN-13	978-1-0716-2524-8 / 9781071625248
Zustand	Neuware