Yeast Functional Genomics
Springer-Verlag New York Inc.
978-1-0716-2256-8 (ISBN)
Cutting edge and practical, Yeast Functional Genomics: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about the evolving field of yeast.
Chapters 1, 9, 16, 20, 22, 24, and 25 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Single-Cell RNA-Sequencing in Yeast using the 10x Genomics Chromium Device.- Genome-Wide Profiling of Transcription Initiation with STRIPE-seq.- A Modified Crosslinking Analysis of cDNAs (CRAC) Protocol for Detecting RNA-Protein Interactions and Transcription at Single Nucleotide Resolution.- High-Resolution Deep Sequencing of Nascent Transcription in Yeast with BioGRO-seq.- Direct Sequencing of RNA and RNA Modification Identification using Nanopore.- UNAGI: Yeast Transcriptome Reconstruction and Gene Discovery using Nanopore Sequencing.- Identification of Taxonomically Restricted Transcripts from Illumina RNA Sequencing Data.- FORK-seq: Single Molecule Profiling of DNA Replication.- CUT&RUN Profiling of the Budding Yeast Epigenome.- ChIP-SICAP: A New Tool to Explore Gene-Regulatory Networks in Candida albicans and Other Yeasts.- Purification of Ribosome-Nascent-Chain Complex for Ribosome Profiling and Selective Ribosome Profiling.- Single-Step Affinity Purification (ssAP) and Mass Spectrometry of Macromolecular Complexes in the Yeast S. cerevisiae.- Composition and Dynamics of Protein Complexes Measured by Quantitative Mass Spectrometry of Affinity Purified Samples.- Deep Mutational Scanning of Protein-Protein Interaction Stability between Partner Expressed from Their Endogenous Loci In Vivo.- Proteomic Mapping by APEX2-Catalyzed Proximity Labeling in Saccharomyces cerevisiae Semi-Permeabilized Cells.- Quantitative Proteomics in Yeast: From bSLIM and Proteome Discoverer Outputs to Graphical Assessment of the Significance of Protein Quantification Scores.- A Strong Cation Exchange Chromatography Protocol for Examining N-Terminal Proteoforms.- RNA Interference (RNAi) as a Tool for High-Resolution Phenotypic Screening of the Pathogenic Yeast Candida glabrata.- High Throughput Gene Mutagenesis Screening using Base Editing.- Saturated Transposon Analysis in Yeast (SATAY) for Deep Functional Mapping of Yeast Genomes.- High-Throughput, High-Precision Colony Phenotyping with Pyphe.- Bulk-Fitness Measurements using Barcode Sequencing Analysis in Yeast.- Prediction of Gene and Genomic Regulation in Candida Species, using the PathoYeastract Database: A Comparative Genomics Approach.- Enabling Studies of Genome-Scale Regulatory Network Evolution in Large Phylogenies with MRTLE.- Omics Analyses, How to Navigate through a Constant Data Deluge.
Erscheinungsdatum | 12.05.2022 |
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Reihe/Serie | Methods in Molecular Biology ; 2477 |
Zusatzinfo | 74 Illustrations, color; 8 Illustrations, black and white; XV, 474 p. 82 illus., 74 illus. in color. With online files/update. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Schlagworte | Chip • COFRADIC • CRISPR-CAS. • Mass Spectrometry • RNA-RNA interactions |
ISBN-10 | 1-0716-2256-0 / 1071622560 |
ISBN-13 | 978-1-0716-2256-8 / 9781071622568 |
Zustand | Neuware |
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