Detection of Cell Death Mechanisms
Springer-Verlag New York Inc.
978-1-0716-1164-7 (ISBN)
Authoritative and accessible, Detection of Cell Death Mechanisms: Methods and Protocols aims to ensure seamless execution of protocols on specific cell death type.
Determination of Caspase Activation by Western Blot.- Detection of Unfolded Protein Response by Polymerase Chain Reaction.- Subcellular Fractionation to Demonstrate Activation of Intrinsic Apoptotic Pathway.- A Triple-Parameter Based Laboratory Friendly Fluorescence Imaging to Identify Apoptosis in Live Cells.- Flow Cytometric Analyses of p53-mediated Cell Cycle Arrest and Apoptosis in Cancer Cells.- A Real-Time, Bioluminescent Apoptosis Assay.- Detection of Anoikis Using Cell Viability Dye and Quantitation of Caspase Activity.- Time and Dose Dependent Toxicity Studies in 3D Cultures Using a Luminescent Lactate Dehydrogenase Assay.- Visualization and Quantification of Neutrophil Extracellular Traps.- 2In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps.- Induction and Detection of Necroptotic Cell Death in Mammalian Cell Culture.- Visualization of Necroptotic Cell Death through Transmission Electron Microscopy.- Methodology for Comprehensive Detection of Pyroptosis.- Determination and Quantitation of Cytotoxic T Cell-Mediated Cell Death.- Detection of Immunogenic Cell Death in Tumor Vaccination Mouse Model.- A Luminescent Assay to Quantify Cell Viability in Real Time.- Kinetic Measurement of Apoptosis and Immune Cell Killing Using Live-Cell Imaging and Analysis.- Two Plasmid-Based Systems for CRISPR/Cas9 Mediated Knockout of Target Genes.- Determining Cell Death Pathway and Regulation by Enrichment Analysis.- Transcription Factor Binding Site Identification and Enrichment Analysis.
Erscheinungsdatum | 17.12.2021 |
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Reihe/Serie | Methods in Molecular Biology ; 2255 |
Zusatzinfo | 60 Illustrations, color; 6 Illustrations, black and white; X, 265 p. 66 illus., 60 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Schlagworte | ApopTag • Apoptosis • Apoptotic Cancer Cells • Bioluminescent Proteasome Assays • Gene expression analysis • Lentiviral Biosensors |
ISBN-10 | 1-0716-1164-X / 107161164X |
ISBN-13 | 978-1-0716-1164-7 / 9781071611647 |
Zustand | Neuware |
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