Optical Tweezers
Springer-Verlag New York Inc.
978-1-0716-2228-5 (ISBN)
Authoritative and practical, Optical Tweezers: Methodsand Protocols, Second Edition serves as an ideal resource for expanding the accessibility and use of optical traps by scientists of diverse disciplines.
The Invention of the Laser.- Art Ashkin and the Origins of Optical Trapping and Particle Manipulation.- Synthesis of Germanium Nanospheres as High Precision Optical Tweezers Probes.- Angular Optical Trapping to Directly Measure DNA Torsional Mechanics.- Implementation of 3D Multi-Color Fluorescence Microscopy in a Quadruple Trap Optical Tweezers System.- One-Dimensional STED Microscopy in Optical Tweezers.- Temperature Quantification and Temperature Control in Optical Tweezers.- High-Resolution Optical Tweezers Combined with Multi-Color Single-Molecule Microscopy.- Generating Negatively Supercoiled DNA Using Dual-Trap Optical Tweezers.- Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay.- Probing the Interaction between Chromatin and Chromatin-Associated Complexes with Optical Tweezers.- Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA Unwinding.- CRISPR/Cas9 On- and Off-Target Activity Using Correlative Force and Fluorescence Single-Molecule Microscopy.- Co-Temporal Single-Molecule Force and Fluorescence Measurements to Determine the Mechanism of Ribosome Translocation.- Using Single-Molecule Optical Tweezers to Study the Conformational Cycle of the Hsp90 Molecular Chaperone.- Tethering Complex Proteins and Protein Complexes for Optical Tweezers Experiments.- Single-Molecule Manipulation Study of Chaperoned SNARE Folding and Assembly with Optical Tweezers.- Using Optical Tweezers to Monitor Allosteric Signals through Changes in Folding Energy Landscapes.- High-Speed Optical Traps Address Dynamics of Processive and Non-Processive Molecular Motors.- Microtubule Dumbbells to Assess the Effect of Force Geometry on Single Kinesin Motors.- Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1A.- Ultra-Fast Force-Clamp Spectroscopy of Microtubule-Binding Proteins.- Catching the Conformational Wave: Measuring the Working Strokesof Protofilaments as They Curl Outward from Disassembling Microtubule Tips.- Mechanics of Single Vimentin Intermediate Filaments under Load.- Quantifying the Interaction Strength between Biopolymers.- Measuring αβ T Cell Receptor-Mediated Mechanosensing Using Optical Tweezers Combined with Fluorescence Imaging.
Erscheinungsdatum | 08.09.2022 |
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Reihe/Serie | Methods in Molecular Biology ; 2478 |
Zusatzinfo | 174 Illustrations, color; 21 Illustrations, black and white; XIV, 757 p. 195 illus., 174 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik |
Naturwissenschaften ► Physik / Astronomie ► Optik | |
Schlagworte | DNA-protein interactions • Mechanosensing • microtubules • motor proteins • optical traps • Protein Folding • Single-molecule experiments |
ISBN-10 | 1-0716-2228-5 / 1071622285 |
ISBN-13 | 978-1-0716-2228-5 / 9781071622285 |
Zustand | Neuware |
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