Notch Signaling Research

Methods and Protocols

Buch | Hardcover

298 Seiten

2022 | 1st ed. 2022
Springer-Verlag New York Inc.
978-1-0716-2200-1 (ISBN)

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This detailed volume explores the large research area of Notch signaling studies in a variety of model organisms. The collected research methods and protocols include the dissection of Notch functional sites, Notch regulators, Notch activity reporters and analysis, roles of Notch in development and diseases, wet and dry lab tools and studies, and bioinformatics analysis. This book particularly emphasizes on the exploration of Notch roles in development and diseases, while acknowledging the importance of combining classic and novel molecular tools and methods, bioinformatics workflows and applications, and different model organisms to fully understand the subject area. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Notch Signaling Research: Methods and Protocols is an ideal guide for researchers in the field, with techniques that could potentially be applied to study the wider world of other signaling pathways as well.

Using the CRISPR/Cas9 System for Dissection of Functional Sites of the Notch Gene in Drosophila melanogaster.- Screening Mutants by Single Fly Genomic PCR.- Generation of Properly Folded Epidermal Growth Factor-Like (EGF) Repeats and Glycosyltransferases Enables In Vitro O-Glycosylation.- Use of FLP/FRT System to Screen for Notch Signaling Regulators in the Drosophila Wing.- High-Throughput Analysis to Identify Activators of Notch Signaling.- Artificial Notch Signaling Activation Method Using Immobilized Ligand Beads.- Mammalian NOTCH Receptors Activation and Signaling Protocols.- Somatic Clonal Analyses Using FLP/FRT and MARCM System to Understand Notch Signaling Mechanism and Its Regulation.- Analyzing the Interaction of RBPJ with Mitotic Chromatin and Its Impact on Transcription Reactivation upon Mitotic Exit.- Assessing the Roles of Potential Notch Signaling Components in Instructive and Permissive Pathways with Two Drosophila Pericardial Reporters.- Image-Based Single-Molecule Analysis of Notch-Dependent Transcription in Its Natural Context.- An Automatic Stage Identification MATLAB Tool to Reveal Notch Expression Pattern in Drosophila Egg Chambers.- Employing the CRISPR Technology for Studying Notch Signaling in the Male Gonad of Drosophila melanogaster.- Immuno-Localization of Notch Signaling in Mouse Preimplantation Embryos.- Studying the NOTCH Signaling Pathway Activation in Kidney Biopsies.- Exosomes as Carriers for Notch Molecules.- In Vivo and Ex Vivo Experimental Approach for Studying Functional Role of Notch in Pulmonary Vascular Disease.- Metastasis Model to Test the Role of Notch Signaling in Prostate Cancer.- Functional Studies of Genetic Variants Associated with Human Diseases in Notch Signaling-Related Genes Using Drosophila.- Bioinformatics Tools to Understand Notch.

Erscheinungsdatum	13.06.2022
Reihe/Serie	Methods in Molecular Biology ; 2472
Zusatzinfo	45 Illustrations, color; 11 Illustrations, black and white; XIII, 298 p. 56 illus., 45 illus. in color. With online files/update.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	dysregulation • Notch disease models • Notch regulators • Notch signaling transduction • receptors • signaling pathways
ISBN-10	1-0716-2200-5 / 1071622005
ISBN-13	978-1-0716-2200-1 / 9781071622001
Zustand	Neuware