Capturing Chromosome Conformation

Methods and Protocols

Beatrice Bodega, Chiara Lanzuolo (Herausgeber)

Buch | Softcover

322 Seiten

2021 | 1st ed. 2021
Springer-Verlag New York Inc.
978-1-0716-0666-7 (ISBN)

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This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Capturing Chromosome Conformation: Methods and Protocols serves as an ideal guide for researchers working to further understand 3D genome organization.

Capturing Chromosome Conformation.- The Chromosome Conformation Capture (3C) in Drosophila melanogaster.- 4C-Seq: Interrogating Chromatin Looping with Circular Chromosome Conformation Capture.- Analysis, Modeling, and Visualization of Chromosome Conformation Capture Experiments.- Targeted DNase Hi-C.- C-HiC: A High Resolution Method for Unbiased Chromatin Conformation Capture Targeting Small Locus.- Computational Analysis of Hi-C Data.- Profiling Chromatin Landscape at High Resolution and Throughput with 2C-ChIP.- Single-Cell DamID to Capture Contacts between DNA and the Nuclear Lamina in Individual Mammalian Cells.- Formaldehyde-Mediated Snapshot of Nuclear Architecture.- Visualization of Chromatin Dynamics by Live Cell Microscopy Using CRISPR/Cas9 Gene Editing and ANCHOR Labeling.- Preparing Map of Chromosome Territories Distribution Frequency.- Higher-Order Chromatin Organization Using 3D DNA Fluorescent In Situ Hybridization.- 3D Immuno-DNA Fluorescence In Situ Hybridization (FISH) for Detection of HIV-1 and Cellular Genes in Primary CD4+ T Cells.- Visualization of Nuclear and Cytoplasmic Long Noncoding RNAs at Single-Cell Level by RNA-FISH.- 3D COMBO chrRNA-DNA-Immuno FISH.- An Algorithm for the Analysis of the 3D Spatial Organization of the Genome.

Erscheinungsdatum	03.09.2021
Reihe/Serie	Methods in Molecular Biology ; 2157
Zusatzinfo	54 Illustrations, color; 12 Illustrations, black and white; XI, 322 p. 66 illus., 54 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Allgemeines / Lexika
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	3D genome organization • Chromosome Conformation Capture • imaging visualization • Next generation sequencing • Nuclear compartmentalization • Tridimensional chromatin conformation
ISBN-10	1-0716-0666-2 / 1071606662
ISBN-13	978-1-0716-0666-7 / 9781071606667
Zustand	Neuware