Zika Virus
Springer-Verlag New York Inc.
978-1-0716-0583-7 (ISBN)
Cutting-edge and practical, Zika Virus: Methods and Protocols is a valuable resource for both novice and expert scientists interested in learning more about this important topic.
Zika Virus: A Brief History and Review of its Pathogenesis Rediscovered.- Zika Virus Isolation, Purification, and Titration.- Evaluating Zika Virus Pathogenesis in Immunocompromised Mice.- Droplet Digital PCR and Immunohistochemistry Techniques to Detect Zika Virus in the Central Nervous System of Mice.- A Plaque Reduction Neutralization Test for the Detection of ZIKV-Specific Antibodies.- Analysis of Serum Anti-Zika Virus Antibodies by Focus Reduction Neutralization Test.- Enzyme-Linked Immunosorbent Assay and Quantitative Reverse Transcription PCR as a Technique to Analyze Inflammation.- Production and Purification of Zika Virus NS1 Glycoprotein in HEK293 Cells.- Optimization of Small-Scale Production of Zika Virus Envelope Glycoprotein by Transient Expression in HEK293 Cells for ELISA.- Zika Virus Peptide ELISA (ZIKV-NS2B-concat ELISA) for Detection of IgG Antibodies to Zika Virus Infection.- Zika Virus Amplification using Strand Displacement Isothermal Method and Sequencing using Nanopore Technology.- Detection of Zika Virus using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP).- Development and Validation of Real-Time RT-LAMP Assays for the Specific Detection of Zika Virus.- Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA.- A Porcine Model of Zika Virus Infection to Profile the In Utero Interferon Alpha Response.- Isolation and Detection of Zika Virus-Infected Rhesus Macaques Lymph Node Cells and Splenocytes.- Discovery of Zika Virus Dependency and Restriction Factors using Flow-Based Arrayed CRISPR Screening for Identification of Targets (FACS-IT).- Zika Virus Targets Multiple Tissues and Cell Types During the First Trimester of Pregnancy.- Use of Primary Human Fetal Astrocytes and Tissue Explants as Ex Vivo Models to Study Zika Virus Infection of the Developing Brain.- Isolation of Monoclonal Antibodies from ZikaVirus-Infected Patient Samples.
Erscheinungsdatum | 14.05.2021 |
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Reihe/Serie | Methods in Molecular Biology ; 2142 |
Zusatzinfo | 42 Illustrations, color; 15 Illustrations, black and white; XII, 290 p. 57 illus., 42 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin |
Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-0716-0583-6 / 1071605836 |
ISBN-13 | 978-1-0716-0583-7 / 9781071605837 |
Zustand | Neuware |
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