Innate Lymphoid Cells

Methods and Protocols

Buch | Softcover

242 Seiten

2021 | 1st ed. 2020
Springer-Verlag New York Inc.
978-1-0716-0340-6 (ISBN)

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This volume explores the latest techniques used to characterize murine and human Innate Lymphoid Cells (ILC) subsets. This book also aims to discuss useful phenotypical and functional assays that may be relevant for studying ILC function in mice and humans. Chapters cover topics such as methods to study microbiome and enteroid cultures; characterizing ILC precursors; and cellular engineering of ILCs for therapeutic use. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Innate Lymphoid Cells: Methods and Protocols is a valuable research tool for anyone interested in ILC biology, and those interested in fundamental immunology, cellular engineering, cell signaling, and clinician scientists.

Protocols for Innate Lymphoid Cell Phenotypic and Functional Characterization: An Overview.- Protocols for Studying Murine ILC Development.- Identification of Murine Innate Lymphoid Cell Subsets in Barrier Tissues and their Draining Lymph Nodes using Flow Cytometry.- Identification and Functional Characterization of Murine Group 3 Innate Lymphoid Cell (ILC3) Subsets in the Intestinal Tract and Associated Lymphoid Tissues.- Identification of Murine and Human Innate Lymphoid Cells in Frozen Tissue Sections using Immunofluorescence.- Assessing Phosphorylation of STAT Transcription Factors in Mouse Innate Lymphoid Cells.- Determining Co-Receptor Expression and Function in Murine ILC2 through Flow Cytometry Characterization and Co-Culture Techniques.- Protocol for Determining the Effect of Neuroendocrine Hormones on Murine ILC Function.- Role of G-Protein Coupled Receptors in Chemotaxis of Innate Lymphoid Cells.- Group 2 ILC Functional Assays inAllergic Airway Inflammation.- Isolation, Culture, and Induction of Plasticity in ILC2s.- Bacterial Infection Allows for Functional Examination of Adoptively Transferred Mouse Innate Lymphoid Cell Subsets.- Isolation, Characterization, and Culture of Intestinal Intraepithelial Lymphocytes.- Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment.- Microbiota Analysis using Sequencing by Synthesis: From Library Preparation to Sequencing.- Establishing Human Intestinal Enteroid/Organoid Lines from Preterm Infant and Adult Tissue.- Characterizing Innate Lymphoid Cell Phenotype and Function in Human Inflammatory Bowel Disease.- CRISPR/Cas9-Based Gene Engineering of Human Natural Killer Cells: Protocols for Knock-Out and Read-Outs to Evaluate the Efficacy.

Erscheinungsdatum	16.03.2021
Reihe/Serie	Methods in Molecular Biology ; 2121
Zusatzinfo	39 Illustrations, color; 9 Illustrations, black and white; XIII, 242 p. 48 illus., 39 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	Allergic inflammation • CD4+ and CD4- lymphoid tissue inducer cells • developmental pathways • Parasitic Helminths • Phenotype Characterization
ISBN-10	1-0716-0340-X / 107160340X
ISBN-13	978-1-0716-0340-6 / 9781071603406
Zustand	Neuware