Reverse Phase Protein Arrays

From Technical and Analytical Fundamentals to Applications

Tesshi Yamada, Satoshi S. Nishizuka, Gordon B. Mills, Lance A. Liotta (Herausgeber)

Buch | Softcover

266 Seiten

2021 | 1st ed. 2019
Springer Verlag, Singapore
978-981-329-757-9 (ISBN)

Artikel merken

This book provides practical guidance on all aspects of reverse phase protein array (RPPA) technology, which permits the quantification of protein levels in cell or tissue lysates. In addition, the latest results are presented from laboratories across the world where experts are successfully running the challenging RPPA data platform. After an introductory chapter by the inventor of the RPPA technique, the demanding task of lysate preparation is explained. Subsequent chapters review printing platforms and analytical platforms and discuss antibody screening in detail. The role of RPPA data in integrative analyses is thoroughly examined, and the various applications of RPPA, for example to identify molecular targeting drugs or evaluate cancer drug efficacy, are discussed with reference to the latest knowledge. It is hoped that the book will foster the implementation of RPPA by documenting the practical details of the technology and by revealing its great potential. It will be of high value for researchers who plan to build an RPPA platform in their institute and for researchers, clinicians, and companies who use RPPA through collaboration with an existing RPPA facility.

Tesshi Yamada, M.D., Ph.D. Professor of International University of Health and Welfare, Narita, Japan Satoshi Nishizuka, M.D., Ph.D. Professor of Iwate Medical University, Japan Gordon Mills, M.D., Ph.D. Professor of University of Texas, MD Anderson Cancer Center Lance Liotta, M.D., Ph.D. Co-Director of the Center for Applied Proteomics and Molecular Medicine, George Mason University

Chapter 1: Origin and future of RPPA technology.- Chapter 2: Lysate preparation.- Chapter 3: Printing platforms 1.- Chapter 4: Printing platforms 2.- Chapter 5: Analytical platforms 1.- Chapter 6: Analytical platforms 2.- Chapter 7: Analytical platforms 3.- Chapter 8. Antibody screening 1.- Chapter 9. Antibody screening 2.- Chapter 10. Antibody screening 3.- Chapter 11. Integrative analysis for genomics and RPPA.- Chapter 12. Integrative analysis of multiple platforms of RPPA.- Chapter 13. Drug screening platforms and RPPA.- Chapter 14. RPPA in small population analysis.- Chapter 15: Molecular target identification using RPPA.- Chapter 16: RPPA in clinical trials.- Chapter 17: RPPA and future medicine.

Erscheinungsdatum	15.01.2021
Reihe/Serie	Advances in Experimental Medicine and Biology ; 1188
Zusatzinfo	60 Illustrations, color; 4 Illustrations, black and white; VIII, 266 p. 64 illus., 60 illus. in color.
Verlagsort	Singapore
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Biochemie
ISBN-10	981-329-757-3 / 9813297573
ISBN-13	978-981-329-757-9 / 9789813297579
Zustand	Neuware