Cereal Genomics

Methods and Protocols

Buch | Softcover

247 Seiten

2020 | 1st ed. 2020
Humana Press Inc. (Verlag)
978-1-4939-9867-8 (ISBN)

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The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cereal Genomics: Methods and Protocols to ensure successful results in the further study of this vital field.

A Low-Cost High-throughput Method for Plant Genomic DNA Isolation.- Cereal Genomics Databases and Plant Genetic Resources in Crop Improvement.- Integrated Genomic Strategies for Cereal Genetic Enhancement: Combining QTL and Association Mapping.- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops.- Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing.- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR.- DNA Methylation and Transcriptomic Next Generation Technologies in Cereal Genomics.- Genome-wide Identification of Regulatory DNA Elements in Crop Plants.- Genome-wide Profiling of Histone Modifications with ChIP-seq.- Whole-genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes.- Genome-wide Identification of Allele-Specific Gene Expression in a Parent‑of‑Origin Specific Manner.- Analysis of Epigenetic Modifications during Vegetative and Reproductive Development in Cereals using Chromatin Immunoprecipitation (ChIP).- Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis.- Emerging Genome Engineering Tools in Crop Research and Breeding.- CRISPR/Cas9-mediated Targeted Mutagenesis in Wheat Doubled Haploids.- Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne.- Agrobacterium Transformation in the Rice Genome.- In vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C4 Photosynthesis in Zea mays.

Erscheinungsdatum	22.09.2020
Reihe/Serie	Methods in Molecular Biology ; 2072
Zusatzinfo	21 Illustrations, color; 9 Illustrations, black and white; XI, 247 p. 30 illus., 21 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Botanik
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	epigenotype-phenotype • Hi-C technique • High-throughput phenotyping • Immunoprecipitation • transcriptomic profiling
ISBN-10	1-4939-9867-6 / 1493998676
ISBN-13	978-1-4939-9867-8 / 9781493998678
Zustand	Neuware